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One-Step Agrobacterium Mediated Transformation of Eight Genes Essential for Rhizobium Symbiotic Signaling Using the Novel Binary Vector System pHUGE

Advancement in plant research is becoming impaired by the fact that the transfer of multiple genes is difficult to achieve. Here we present a new binary vector for Agrobacterium tumefaciens mediated transformation, pHUGE-Red, in concert with a cloning strategy suited for the transfer of up to nine g...

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Detalles Bibliográficos
Autores principales:	Untergasser, Andreas, Bijl, Gerben J. M., Liu, Wei, Bisseling, Ton, Schaart, Jan G., Geurts, René
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480454/ https://www.ncbi.nlm.nih.gov/pubmed/23112864 http://dx.doi.org/10.1371/journal.pone.0047885

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