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The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins
Cytoplasmic microtubules exist as distinct dynamic and stable populations within the cell. Stable microtubules direct and maintain cell polarity and it is thought that their stabilization is dependent on coordinative organization between the microtubule network and the actin cytoskeleton. A growing...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480493/ https://www.ncbi.nlm.nih.gov/pubmed/23110170 http://dx.doi.org/10.1371/journal.pone.0048041 |
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author | Thurston, Susan F. Kulacz, Wojciech A. Shaikh, Sahir Lee, Jonathan M. Copeland, John W. |
author_facet | Thurston, Susan F. Kulacz, Wojciech A. Shaikh, Sahir Lee, Jonathan M. Copeland, John W. |
author_sort | Thurston, Susan F. |
collection | PubMed |
description | Cytoplasmic microtubules exist as distinct dynamic and stable populations within the cell. Stable microtubules direct and maintain cell polarity and it is thought that their stabilization is dependent on coordinative organization between the microtubule network and the actin cytoskeleton. A growing body of work suggests that some members of the formin family of actin remodeling proteins also regulate microtubule organization and stability. For example, we showed previously that expression of the novel formin INF1 is sufficient to induce microtubule stabilization and tubulin acetylation, but not tubulin detyrosination. An important issue with respect to the relationship between formins and microtubules is the determination of which formin domains mediate microtubule stabilization. INF1 has a distinct microtubule-binding domain at its C-terminus and the endogenous INF1 protein is associated with the microtubule network. Surprisingly, the INF1 microtubule-binding domain is not essential for INF1-induced microtubule acetylation. We show here that expression of the isolated FH1 + FH2 functional unit of INF1 is sufficient to induce microtubule acetylation independent of the INF1 microtubule-binding domain. It is not yet clear whether or not microtubule stabilization is a general property of all mammalian formins; therefore we expressed constitutively active derivatives of thirteen of the fifteen mammalian formin proteins in HeLa and NIH3T3 cells and measured their effects on stress fiber formation, MT organization and MT acetylation. We found that expression of the FH1 + FH2 unit of the majority of mammalian formins is sufficient to induce microtubule acetylation. Our results suggest that the regulation of microtubule acetylation is likely a general formin activity and that the FH2 should be thought of as a dual-function domain capable of regulating both actin and microtubule networks. |
format | Online Article Text |
id | pubmed-3480493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34804932012-10-29 The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins Thurston, Susan F. Kulacz, Wojciech A. Shaikh, Sahir Lee, Jonathan M. Copeland, John W. PLoS One Research Article Cytoplasmic microtubules exist as distinct dynamic and stable populations within the cell. Stable microtubules direct and maintain cell polarity and it is thought that their stabilization is dependent on coordinative organization between the microtubule network and the actin cytoskeleton. A growing body of work suggests that some members of the formin family of actin remodeling proteins also regulate microtubule organization and stability. For example, we showed previously that expression of the novel formin INF1 is sufficient to induce microtubule stabilization and tubulin acetylation, but not tubulin detyrosination. An important issue with respect to the relationship between formins and microtubules is the determination of which formin domains mediate microtubule stabilization. INF1 has a distinct microtubule-binding domain at its C-terminus and the endogenous INF1 protein is associated with the microtubule network. Surprisingly, the INF1 microtubule-binding domain is not essential for INF1-induced microtubule acetylation. We show here that expression of the isolated FH1 + FH2 functional unit of INF1 is sufficient to induce microtubule acetylation independent of the INF1 microtubule-binding domain. It is not yet clear whether or not microtubule stabilization is a general property of all mammalian formins; therefore we expressed constitutively active derivatives of thirteen of the fifteen mammalian formin proteins in HeLa and NIH3T3 cells and measured their effects on stress fiber formation, MT organization and MT acetylation. We found that expression of the FH1 + FH2 unit of the majority of mammalian formins is sufficient to induce microtubule acetylation. Our results suggest that the regulation of microtubule acetylation is likely a general formin activity and that the FH2 should be thought of as a dual-function domain capable of regulating both actin and microtubule networks. Public Library of Science 2012-10-24 /pmc/articles/PMC3480493/ /pubmed/23110170 http://dx.doi.org/10.1371/journal.pone.0048041 Text en © 2012 Thurston et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Thurston, Susan F. Kulacz, Wojciech A. Shaikh, Sahir Lee, Jonathan M. Copeland, John W. The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins |
title | The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins |
title_full | The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins |
title_fullStr | The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins |
title_full_unstemmed | The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins |
title_short | The Ability to Induce Microtubule Acetylation Is a General Feature of Formin Proteins |
title_sort | ability to induce microtubule acetylation is a general feature of formin proteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480493/ https://www.ncbi.nlm.nih.gov/pubmed/23110170 http://dx.doi.org/10.1371/journal.pone.0048041 |
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