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Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit

Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes...

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Autores principales: Vagnarelli, Paola, Ribeiro, Susana, Sennels, Lau, Sanchez-Pulido, Luis, de Lima Alves, Flavia, Verheyen, Toon, Kelly, David A., Ponting, Chris P., Rappsilber, Juri, Earnshaw, William C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480639/
https://www.ncbi.nlm.nih.gov/pubmed/21820363
http://dx.doi.org/10.1016/j.devcel.2011.06.020
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author Vagnarelli, Paola
Ribeiro, Susana
Sennels, Lau
Sanchez-Pulido, Luis
de Lima Alves, Flavia
Verheyen, Toon
Kelly, David A.
Ponting, Chris P.
Rappsilber, Juri
Earnshaw, William C.
author_facet Vagnarelli, Paola
Ribeiro, Susana
Sennels, Lau
Sanchez-Pulido, Luis
de Lima Alves, Flavia
Verheyen, Toon
Kelly, David A.
Ponting, Chris P.
Rappsilber, Juri
Earnshaw, William C.
author_sort Vagnarelli, Paola
collection PubMed
description Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain localizes Repo-Man to bulk chromatin in early anaphase. There, it targets PP1 for the dephosphorylation of histone H3 and possibly other chromosomal substrates. An N-terminal domain localizes Repo-Man to the chromosome periphery later in anaphase. There, it is responsible for the recruitment of nuclear components such as Importin β and Nup153 in a PP1-independent manner. These observations identify Repo-Man as a key factor that coordinates chromatin remodeling and early events of nuclear envelope reformation during mitotic exit.
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spelling pubmed-34806392012-11-14 Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit Vagnarelli, Paola Ribeiro, Susana Sennels, Lau Sanchez-Pulido, Luis de Lima Alves, Flavia Verheyen, Toon Kelly, David A. Ponting, Chris P. Rappsilber, Juri Earnshaw, William C. Dev Cell Article Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain localizes Repo-Man to bulk chromatin in early anaphase. There, it targets PP1 for the dephosphorylation of histone H3 and possibly other chromosomal substrates. An N-terminal domain localizes Repo-Man to the chromosome periphery later in anaphase. There, it is responsible for the recruitment of nuclear components such as Importin β and Nup153 in a PP1-independent manner. These observations identify Repo-Man as a key factor that coordinates chromatin remodeling and early events of nuclear envelope reformation during mitotic exit. Cell Press 2011-08-16 /pmc/articles/PMC3480639/ /pubmed/21820363 http://dx.doi.org/10.1016/j.devcel.2011.06.020 Text en © 2011 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Vagnarelli, Paola
Ribeiro, Susana
Sennels, Lau
Sanchez-Pulido, Luis
de Lima Alves, Flavia
Verheyen, Toon
Kelly, David A.
Ponting, Chris P.
Rappsilber, Juri
Earnshaw, William C.
Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit
title Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit
title_full Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit
title_fullStr Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit
title_full_unstemmed Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit
title_short Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit
title_sort repo-man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480639/
https://www.ncbi.nlm.nih.gov/pubmed/21820363
http://dx.doi.org/10.1016/j.devcel.2011.06.020
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