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Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis

This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or...

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Autores principales: Zhao, Xin, Jia, Hongmei, Yang, Shijun, Liu, Yuetao, Deng, Bo, Xu, Xueyan, Zhang, Tao, Zhou, Hang, Zu, Chengzhe, Yin, He, Li, Ting, Song, Yijun, Wang, Yueqi, Li, Pengtao, Zou, Zhongmei, Cai, Dayong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480689/
https://www.ncbi.nlm.nih.gov/pubmed/23118797
http://dx.doi.org/10.1155/2012/786365
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author Zhao, Xin
Jia, Hongmei
Yang, Shijun
Liu, Yuetao
Deng, Bo
Xu, Xueyan
Zhang, Tao
Zhou, Hang
Zu, Chengzhe
Yin, He
Li, Ting
Song, Yijun
Wang, Yueqi
Li, Pengtao
Zou, Zhongmei
Cai, Dayong
author_facet Zhao, Xin
Jia, Hongmei
Yang, Shijun
Liu, Yuetao
Deng, Bo
Xu, Xueyan
Zhang, Tao
Zhou, Hang
Zu, Chengzhe
Yin, He
Li, Ting
Song, Yijun
Wang, Yueqi
Li, Pengtao
Zou, Zhongmei
Cai, Dayong
author_sort Zhao, Xin
collection PubMed
description This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or heme oxygenase-1 (HO-1) in portal triads was assessed. The isolated portal perfused rat liver (IPPRL) was performed at d (0), d (28), d (56) , and d (84) in the progression of chronic hepatitis. After constricting with phenylephrine, the portal veins were relaxed with Sal B. The EC(50) of Sal B for relaxing portal veins was −2.04 × 10(−9), 7.28 × 10(−11), 1.52 × 10(−11), and 8.44 × 10(−11) mol/L at d (0), d (28), d (56), and d (84), respectively. More macrophages infiltrated in portal triads and expressed more iNOS or HO-1 as PH advanced. The areas under the curve (AUCs) of Sal B for reducing PH were positively correlated with the levels of iNOS or HO-1 in portal triads, and so did with serum CD163 levels. Sal B reduces PH in IPPRL with chronic hepatitis, via promoting portal relaxation due to macrophage-originated NO or CO in portal triads, partly at least.
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spelling pubmed-34806892012-11-01 Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis Zhao, Xin Jia, Hongmei Yang, Shijun Liu, Yuetao Deng, Bo Xu, Xueyan Zhang, Tao Zhou, Hang Zu, Chengzhe Yin, He Li, Ting Song, Yijun Wang, Yueqi Li, Pengtao Zou, Zhongmei Cai, Dayong Evid Based Complement Alternat Med Research Article This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or heme oxygenase-1 (HO-1) in portal triads was assessed. The isolated portal perfused rat liver (IPPRL) was performed at d (0), d (28), d (56) , and d (84) in the progression of chronic hepatitis. After constricting with phenylephrine, the portal veins were relaxed with Sal B. The EC(50) of Sal B for relaxing portal veins was −2.04 × 10(−9), 7.28 × 10(−11), 1.52 × 10(−11), and 8.44 × 10(−11) mol/L at d (0), d (28), d (56), and d (84), respectively. More macrophages infiltrated in portal triads and expressed more iNOS or HO-1 as PH advanced. The areas under the curve (AUCs) of Sal B for reducing PH were positively correlated with the levels of iNOS or HO-1 in portal triads, and so did with serum CD163 levels. Sal B reduces PH in IPPRL with chronic hepatitis, via promoting portal relaxation due to macrophage-originated NO or CO in portal triads, partly at least. Hindawi Publishing Corporation 2012 2012-10-16 /pmc/articles/PMC3480689/ /pubmed/23118797 http://dx.doi.org/10.1155/2012/786365 Text en Copyright © 2012 Xin Zhao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Xin
Jia, Hongmei
Yang, Shijun
Liu, Yuetao
Deng, Bo
Xu, Xueyan
Zhang, Tao
Zhou, Hang
Zu, Chengzhe
Yin, He
Li, Ting
Song, Yijun
Wang, Yueqi
Li, Pengtao
Zou, Zhongmei
Cai, Dayong
Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
title Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
title_full Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
title_fullStr Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
title_full_unstemmed Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
title_short Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
title_sort salvianolic acid b reducing portal hypertension depends on macrophages in isolated portal perfused rat livers with chronic hepatitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480689/
https://www.ncbi.nlm.nih.gov/pubmed/23118797
http://dx.doi.org/10.1155/2012/786365
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