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Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis
This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480689/ https://www.ncbi.nlm.nih.gov/pubmed/23118797 http://dx.doi.org/10.1155/2012/786365 |
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author | Zhao, Xin Jia, Hongmei Yang, Shijun Liu, Yuetao Deng, Bo Xu, Xueyan Zhang, Tao Zhou, Hang Zu, Chengzhe Yin, He Li, Ting Song, Yijun Wang, Yueqi Li, Pengtao Zou, Zhongmei Cai, Dayong |
author_facet | Zhao, Xin Jia, Hongmei Yang, Shijun Liu, Yuetao Deng, Bo Xu, Xueyan Zhang, Tao Zhou, Hang Zu, Chengzhe Yin, He Li, Ting Song, Yijun Wang, Yueqi Li, Pengtao Zou, Zhongmei Cai, Dayong |
author_sort | Zhao, Xin |
collection | PubMed |
description | This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or heme oxygenase-1 (HO-1) in portal triads was assessed. The isolated portal perfused rat liver (IPPRL) was performed at d (0), d (28), d (56) , and d (84) in the progression of chronic hepatitis. After constricting with phenylephrine, the portal veins were relaxed with Sal B. The EC(50) of Sal B for relaxing portal veins was −2.04 × 10(−9), 7.28 × 10(−11), 1.52 × 10(−11), and 8.44 × 10(−11) mol/L at d (0), d (28), d (56), and d (84), respectively. More macrophages infiltrated in portal triads and expressed more iNOS or HO-1 as PH advanced. The areas under the curve (AUCs) of Sal B for reducing PH were positively correlated with the levels of iNOS or HO-1 in portal triads, and so did with serum CD163 levels. Sal B reduces PH in IPPRL with chronic hepatitis, via promoting portal relaxation due to macrophage-originated NO or CO in portal triads, partly at least. |
format | Online Article Text |
id | pubmed-3480689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34806892012-11-01 Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis Zhao, Xin Jia, Hongmei Yang, Shijun Liu, Yuetao Deng, Bo Xu, Xueyan Zhang, Tao Zhou, Hang Zu, Chengzhe Yin, He Li, Ting Song, Yijun Wang, Yueqi Li, Pengtao Zou, Zhongmei Cai, Dayong Evid Based Complement Alternat Med Research Article This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or heme oxygenase-1 (HO-1) in portal triads was assessed. The isolated portal perfused rat liver (IPPRL) was performed at d (0), d (28), d (56) , and d (84) in the progression of chronic hepatitis. After constricting with phenylephrine, the portal veins were relaxed with Sal B. The EC(50) of Sal B for relaxing portal veins was −2.04 × 10(−9), 7.28 × 10(−11), 1.52 × 10(−11), and 8.44 × 10(−11) mol/L at d (0), d (28), d (56), and d (84), respectively. More macrophages infiltrated in portal triads and expressed more iNOS or HO-1 as PH advanced. The areas under the curve (AUCs) of Sal B for reducing PH were positively correlated with the levels of iNOS or HO-1 in portal triads, and so did with serum CD163 levels. Sal B reduces PH in IPPRL with chronic hepatitis, via promoting portal relaxation due to macrophage-originated NO or CO in portal triads, partly at least. Hindawi Publishing Corporation 2012 2012-10-16 /pmc/articles/PMC3480689/ /pubmed/23118797 http://dx.doi.org/10.1155/2012/786365 Text en Copyright © 2012 Xin Zhao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhao, Xin Jia, Hongmei Yang, Shijun Liu, Yuetao Deng, Bo Xu, Xueyan Zhang, Tao Zhou, Hang Zu, Chengzhe Yin, He Li, Ting Song, Yijun Wang, Yueqi Li, Pengtao Zou, Zhongmei Cai, Dayong Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis |
title | Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis |
title_full | Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis |
title_fullStr | Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis |
title_full_unstemmed | Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis |
title_short | Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis |
title_sort | salvianolic acid b reducing portal hypertension depends on macrophages in isolated portal perfused rat livers with chronic hepatitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480689/ https://www.ncbi.nlm.nih.gov/pubmed/23118797 http://dx.doi.org/10.1155/2012/786365 |
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