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Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations

A simple, specific, accurate and stability-indicating UV- Spectrophotometric method was developed for the estimation of candesartan cilexitil, using a Shimadzu, model 1700 spectrophotometer and a mobile phase composed of methanol: water in the ratio of 9:1 at wave length (λ(max)) 254 nm. Linearity w...

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Autores principales: Pradhan, K. K., Mishra, U. S., Pattnaik, S., Panda, C. K., Sahu, K. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480759/
https://www.ncbi.nlm.nih.gov/pubmed/23112408
http://dx.doi.org/10.4103/0250-474X.100254
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author Pradhan, K. K.
Mishra, U. S.
Pattnaik, S.
Panda, C. K.
Sahu, K. C.
author_facet Pradhan, K. K.
Mishra, U. S.
Pattnaik, S.
Panda, C. K.
Sahu, K. C.
author_sort Pradhan, K. K.
collection PubMed
description A simple, specific, accurate and stability-indicating UV- Spectrophotometric method was developed for the estimation of candesartan cilexitil, using a Shimadzu, model 1700 spectrophotometer and a mobile phase composed of methanol: water in the ratio of 9:1 at wave length (λ(max)) 254 nm. Linearity was established for candesartan in the range of 10-90 μg/ml. The percentage recovery of was found to be in the range of 99.76-100.79%. The drug was subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, UV light and photolytic degradation. Validation experiments performed to demonstrate system suitability, specificity, precision, linearity, accuracy, interday assay, intraday assay, robustness, ruggedness, LOD, and LOQ. While estimating the commercial formulation there was no interference of excipients and other additives. Hence this method can be used for routine determination of candesartan cilexetil in bulk and their pharmaceutical dosage forms. The proposed method for stability study shows that there was appreciable degradation found in stress condition of candesartan.
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spelling pubmed-34807592012-10-30 Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations Pradhan, K. K. Mishra, U. S. Pattnaik, S. Panda, C. K. Sahu, K. C. Indian J Pharm Sci Short Communications A simple, specific, accurate and stability-indicating UV- Spectrophotometric method was developed for the estimation of candesartan cilexitil, using a Shimadzu, model 1700 spectrophotometer and a mobile phase composed of methanol: water in the ratio of 9:1 at wave length (λ(max)) 254 nm. Linearity was established for candesartan in the range of 10-90 μg/ml. The percentage recovery of was found to be in the range of 99.76-100.79%. The drug was subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, UV light and photolytic degradation. Validation experiments performed to demonstrate system suitability, specificity, precision, linearity, accuracy, interday assay, intraday assay, robustness, ruggedness, LOD, and LOQ. While estimating the commercial formulation there was no interference of excipients and other additives. Hence this method can be used for routine determination of candesartan cilexetil in bulk and their pharmaceutical dosage forms. The proposed method for stability study shows that there was appreciable degradation found in stress condition of candesartan. Medknow Publications & Media Pvt Ltd 2011 /pmc/articles/PMC3480759/ /pubmed/23112408 http://dx.doi.org/10.4103/0250-474X.100254 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Pradhan, K. K.
Mishra, U. S.
Pattnaik, S.
Panda, C. K.
Sahu, K. C.
Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations
title Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations
title_full Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations
title_fullStr Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations
title_full_unstemmed Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations
title_short Development and Validation of a Stability-indicating UV Spectroscopic Method for Candesartan in Bulk and Formulations
title_sort development and validation of a stability-indicating uv spectroscopic method for candesartan in bulk and formulations
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480759/
https://www.ncbi.nlm.nih.gov/pubmed/23112408
http://dx.doi.org/10.4103/0250-474X.100254
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