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Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development

BACKGROUNDS: Previous studies suggested that endocrine disruptors (ED) are toxic on preimplantation embryos and inhibit development of embryos in vitro culture. However, information about the toxicity of endocrine disruptors on preimplantation development of embryo in human reproductive environment...

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Autores principales: Lee, Myeong-Seop, Lee, Young-Sang, Lee, Hae-Hyeog, Song, Ho-Yeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480945/
https://www.ncbi.nlm.nih.gov/pubmed/22546201
http://dx.doi.org/10.1186/1745-6673-7-7
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author Lee, Myeong-Seop
Lee, Young-Sang
Lee, Hae-Hyeog
Song, Ho-Yeon
author_facet Lee, Myeong-Seop
Lee, Young-Sang
Lee, Hae-Hyeog
Song, Ho-Yeon
author_sort Lee, Myeong-Seop
collection PubMed
description BACKGROUNDS: Previous studies suggested that endocrine disruptors (ED) are toxic on preimplantation embryos and inhibit development of embryos in vitro culture. However, information about the toxicity of endocrine disruptors on preimplantation development of embryo in human reproductive environment is lacking. METHODS: Bisphenol A (BPA) and Aroclor 1254 (polychlorinated biphenyls) were used as endocrine disruptors in this study. Mouse 2-cell embryos were cultured in medium alone or vehicle or co-cultured with human endometrial epithelial layers in increasing ED concentrations. RESULTS: At 72 hours the percentage of normal blastocyst were decreased by ED in a dose-dependent manner while the co-culture system significantly enhanced the rate and reduced the toxicity of endocrine disruptors on the embryonic development in vitro. CONCLUSIONS: In conclusion, although EDs have the toxic effect on embryo development, the co-culture with human endometrial cell reduced the preimplantation embryo from it thereby making human reproductive environment protective to preimplantation embryo from the toxicity of endocrine disruptors.
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spelling pubmed-34809452012-10-27 Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development Lee, Myeong-Seop Lee, Young-Sang Lee, Hae-Hyeog Song, Ho-Yeon J Occup Med Toxicol Research BACKGROUNDS: Previous studies suggested that endocrine disruptors (ED) are toxic on preimplantation embryos and inhibit development of embryos in vitro culture. However, information about the toxicity of endocrine disruptors on preimplantation development of embryo in human reproductive environment is lacking. METHODS: Bisphenol A (BPA) and Aroclor 1254 (polychlorinated biphenyls) were used as endocrine disruptors in this study. Mouse 2-cell embryos were cultured in medium alone or vehicle or co-cultured with human endometrial epithelial layers in increasing ED concentrations. RESULTS: At 72 hours the percentage of normal blastocyst were decreased by ED in a dose-dependent manner while the co-culture system significantly enhanced the rate and reduced the toxicity of endocrine disruptors on the embryonic development in vitro. CONCLUSIONS: In conclusion, although EDs have the toxic effect on embryo development, the co-culture with human endometrial cell reduced the preimplantation embryo from it thereby making human reproductive environment protective to preimplantation embryo from the toxicity of endocrine disruptors. BioMed Central 2012-04-30 /pmc/articles/PMC3480945/ /pubmed/22546201 http://dx.doi.org/10.1186/1745-6673-7-7 Text en Copyright ©2012 Lee et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lee, Myeong-Seop
Lee, Young-Sang
Lee, Hae-Hyeog
Song, Ho-Yeon
Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
title Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
title_full Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
title_fullStr Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
title_full_unstemmed Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
title_short Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
title_sort human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480945/
https://www.ncbi.nlm.nih.gov/pubmed/22546201
http://dx.doi.org/10.1186/1745-6673-7-7
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