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A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3481011/ https://www.ncbi.nlm.nih.gov/pubmed/22825454 http://dx.doi.org/10.3892/ijo.2012.1556 |
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author | WAN, YING-WOOI RAESE, REBECCA A. FORTNEY, JAMES E. XIAO, CHANGCHANG LUO, DAJIE CAVENDISH, JOHN GIBSON, LAURA F. CASTRANOVA, VINCENT QIAN, YONG GUO, NANCY LAN |
author_facet | WAN, YING-WOOI RAESE, REBECCA A. FORTNEY, JAMES E. XIAO, CHANGCHANG LUO, DAJIE CAVENDISH, JOHN GIBSON, LAURA F. CASTRANOVA, VINCENT QIAN, YONG GUO, NANCY LAN |
author_sort | WAN, YING-WOOI |
collection | PubMed |
description | Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple signaling pathways was modeled among a set of genes associated with smoking and lung cancer survival. This approach identified and validated a 7-gene signature for lung cancer diagnosis and prognosis in smokers using patient transcriptional profiles (n=847). The smoking-associated gene coexpression networks in lung adenocarcinoma tumors (n=442) were highly significant in terms of biological relevance (network precision = 0.91, FDR<0.01) when evaluated with numerous databases containing multi-level molecular associations. The gene coexpression network in smoking lung adenocarcinoma patients was confirmed in qRT-PCR assays of the identified biomarkers and involved signaling pathway genes in human lung adenocarcinoma cells (H23) treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Furthermore, the western blotting results of p53, phospho-p53, Rb and EGFR in NNK-treated H23 and transformed normal human lung epithelial cells (BEAS-2B) support their functional involvement in smoking-induced lung cancer carcinogenesis and progression. |
format | Online Article Text |
id | pubmed-3481011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-34810112013-03-04 A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis WAN, YING-WOOI RAESE, REBECCA A. FORTNEY, JAMES E. XIAO, CHANGCHANG LUO, DAJIE CAVENDISH, JOHN GIBSON, LAURA F. CASTRANOVA, VINCENT QIAN, YONG GUO, NANCY LAN Int J Oncol Articles Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple signaling pathways was modeled among a set of genes associated with smoking and lung cancer survival. This approach identified and validated a 7-gene signature for lung cancer diagnosis and prognosis in smokers using patient transcriptional profiles (n=847). The smoking-associated gene coexpression networks in lung adenocarcinoma tumors (n=442) were highly significant in terms of biological relevance (network precision = 0.91, FDR<0.01) when evaluated with numerous databases containing multi-level molecular associations. The gene coexpression network in smoking lung adenocarcinoma patients was confirmed in qRT-PCR assays of the identified biomarkers and involved signaling pathway genes in human lung adenocarcinoma cells (H23) treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Furthermore, the western blotting results of p53, phospho-p53, Rb and EGFR in NNK-treated H23 and transformed normal human lung epithelial cells (BEAS-2B) support their functional involvement in smoking-induced lung cancer carcinogenesis and progression. D.A. Spandidos 2012-07-16 /pmc/articles/PMC3481011/ /pubmed/22825454 http://dx.doi.org/10.3892/ijo.2012.1556 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles WAN, YING-WOOI RAESE, REBECCA A. FORTNEY, JAMES E. XIAO, CHANGCHANG LUO, DAJIE CAVENDISH, JOHN GIBSON, LAURA F. CASTRANOVA, VINCENT QIAN, YONG GUO, NANCY LAN A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
title | A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
title_full | A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
title_fullStr | A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
title_full_unstemmed | A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
title_short | A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
title_sort | smoking-associated 7-gene signature for lung cancer diagnosis and prognosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3481011/ https://www.ncbi.nlm.nih.gov/pubmed/22825454 http://dx.doi.org/10.3892/ijo.2012.1556 |
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