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A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis

Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple...

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Autores principales: WAN, YING-WOOI, RAESE, REBECCA A., FORTNEY, JAMES E., XIAO, CHANGCHANG, LUO, DAJIE, CAVENDISH, JOHN, GIBSON, LAURA F., CASTRANOVA, VINCENT, QIAN, YONG, GUO, NANCY LAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3481011/
https://www.ncbi.nlm.nih.gov/pubmed/22825454
http://dx.doi.org/10.3892/ijo.2012.1556
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author WAN, YING-WOOI
RAESE, REBECCA A.
FORTNEY, JAMES E.
XIAO, CHANGCHANG
LUO, DAJIE
CAVENDISH, JOHN
GIBSON, LAURA F.
CASTRANOVA, VINCENT
QIAN, YONG
GUO, NANCY LAN
author_facet WAN, YING-WOOI
RAESE, REBECCA A.
FORTNEY, JAMES E.
XIAO, CHANGCHANG
LUO, DAJIE
CAVENDISH, JOHN
GIBSON, LAURA F.
CASTRANOVA, VINCENT
QIAN, YONG
GUO, NANCY LAN
author_sort WAN, YING-WOOI
collection PubMed
description Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple signaling pathways was modeled among a set of genes associated with smoking and lung cancer survival. This approach identified and validated a 7-gene signature for lung cancer diagnosis and prognosis in smokers using patient transcriptional profiles (n=847). The smoking-associated gene coexpression networks in lung adenocarcinoma tumors (n=442) were highly significant in terms of biological relevance (network precision = 0.91, FDR<0.01) when evaluated with numerous databases containing multi-level molecular associations. The gene coexpression network in smoking lung adenocarcinoma patients was confirmed in qRT-PCR assays of the identified biomarkers and involved signaling pathway genes in human lung adenocarcinoma cells (H23) treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Furthermore, the western blotting results of p53, phospho-p53, Rb and EGFR in NNK-treated H23 and transformed normal human lung epithelial cells (BEAS-2B) support their functional involvement in smoking-induced lung cancer carcinogenesis and progression.
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spelling pubmed-34810112013-03-04 A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis WAN, YING-WOOI RAESE, REBECCA A. FORTNEY, JAMES E. XIAO, CHANGCHANG LUO, DAJIE CAVENDISH, JOHN GIBSON, LAURA F. CASTRANOVA, VINCENT QIAN, YONG GUO, NANCY LAN Int J Oncol Articles Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple signaling pathways was modeled among a set of genes associated with smoking and lung cancer survival. This approach identified and validated a 7-gene signature for lung cancer diagnosis and prognosis in smokers using patient transcriptional profiles (n=847). The smoking-associated gene coexpression networks in lung adenocarcinoma tumors (n=442) were highly significant in terms of biological relevance (network precision = 0.91, FDR<0.01) when evaluated with numerous databases containing multi-level molecular associations. The gene coexpression network in smoking lung adenocarcinoma patients was confirmed in qRT-PCR assays of the identified biomarkers and involved signaling pathway genes in human lung adenocarcinoma cells (H23) treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Furthermore, the western blotting results of p53, phospho-p53, Rb and EGFR in NNK-treated H23 and transformed normal human lung epithelial cells (BEAS-2B) support their functional involvement in smoking-induced lung cancer carcinogenesis and progression. D.A. Spandidos 2012-07-16 /pmc/articles/PMC3481011/ /pubmed/22825454 http://dx.doi.org/10.3892/ijo.2012.1556 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WAN, YING-WOOI
RAESE, REBECCA A.
FORTNEY, JAMES E.
XIAO, CHANGCHANG
LUO, DAJIE
CAVENDISH, JOHN
GIBSON, LAURA F.
CASTRANOVA, VINCENT
QIAN, YONG
GUO, NANCY LAN
A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
title A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
title_full A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
title_fullStr A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
title_full_unstemmed A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
title_short A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
title_sort smoking-associated 7-gene signature for lung cancer diagnosis and prognosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3481011/
https://www.ncbi.nlm.nih.gov/pubmed/22825454
http://dx.doi.org/10.3892/ijo.2012.1556
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