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Autoinducers Act as Biological Timers in Vibrio harveyi

Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, p...

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Autores principales: Anetzberger, Claudia, Reiger, Matthias, Fekete, Agnes, Schell, Ursula, Stambrau, Nina, Plener, Laure, Kopka, Joachim, Schmitt-Kopplin, Phillippe, Hilbi, Hubert, Jung, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482212/
https://www.ncbi.nlm.nih.gov/pubmed/23110227
http://dx.doi.org/10.1371/journal.pone.0048310
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author Anetzberger, Claudia
Reiger, Matthias
Fekete, Agnes
Schell, Ursula
Stambrau, Nina
Plener, Laure
Kopka, Joachim
Schmitt-Kopplin, Phillippe
Hilbi, Hubert
Jung, Kirsten
author_facet Anetzberger, Claudia
Reiger, Matthias
Fekete, Agnes
Schell, Ursula
Stambrau, Nina
Plener, Laure
Kopka, Joachim
Schmitt-Kopplin, Phillippe
Hilbi, Hubert
Jung, Kirsten
author_sort Anetzberger, Claudia
collection PubMed
description Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1, AI-2 and CAI-1 are recognized by different receptors, but all information is channeled into the same signaling cascade, which controls a specific set of genes. Here we examine temporal variations of availability and concentration of the three autoinducers in V. harveyi, and monitor the phenotypes they regulate, from the early exponential to the stationary growth phase in liquid culture. Specifically, the exponential growth phase is characterized by an increase in AI-2 and the induction of bioluminescence, while HAI-1 and CAI-1 are undetectable prior to the late exponential growth phase. CAI-1 activity reaches its maximum upon entry into stationary phase, while molar concentrations of AI-2 and HAI-1 become approximately equal. Similarly, autoinducer-dependent exoproteolytic activity increases at the transition into stationary phase. These findings are reflected in temporal alterations in expression of the luxR gene that encodes the master regulator LuxR, and of four autoinducer-regulated genes during growth. Moreover, in vitro phosphorylation assays reveal a tight correlation between the HAI-1/AI-2 ratio as input and levels of receptor-mediated phosphorylation of LuxU as output. Our study supports a model in which the combinations of autoinducers available, rather than cell density per se, determine the timing of various processes in V. harveyi populations.
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spelling pubmed-34822122012-10-29 Autoinducers Act as Biological Timers in Vibrio harveyi Anetzberger, Claudia Reiger, Matthias Fekete, Agnes Schell, Ursula Stambrau, Nina Plener, Laure Kopka, Joachim Schmitt-Kopplin, Phillippe Hilbi, Hubert Jung, Kirsten PLoS One Research Article Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1, AI-2 and CAI-1 are recognized by different receptors, but all information is channeled into the same signaling cascade, which controls a specific set of genes. Here we examine temporal variations of availability and concentration of the three autoinducers in V. harveyi, and monitor the phenotypes they regulate, from the early exponential to the stationary growth phase in liquid culture. Specifically, the exponential growth phase is characterized by an increase in AI-2 and the induction of bioluminescence, while HAI-1 and CAI-1 are undetectable prior to the late exponential growth phase. CAI-1 activity reaches its maximum upon entry into stationary phase, while molar concentrations of AI-2 and HAI-1 become approximately equal. Similarly, autoinducer-dependent exoproteolytic activity increases at the transition into stationary phase. These findings are reflected in temporal alterations in expression of the luxR gene that encodes the master regulator LuxR, and of four autoinducer-regulated genes during growth. Moreover, in vitro phosphorylation assays reveal a tight correlation between the HAI-1/AI-2 ratio as input and levels of receptor-mediated phosphorylation of LuxU as output. Our study supports a model in which the combinations of autoinducers available, rather than cell density per se, determine the timing of various processes in V. harveyi populations. Public Library of Science 2012-10-26 /pmc/articles/PMC3482212/ /pubmed/23110227 http://dx.doi.org/10.1371/journal.pone.0048310 Text en © 2012 Anetzberger et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Anetzberger, Claudia
Reiger, Matthias
Fekete, Agnes
Schell, Ursula
Stambrau, Nina
Plener, Laure
Kopka, Joachim
Schmitt-Kopplin, Phillippe
Hilbi, Hubert
Jung, Kirsten
Autoinducers Act as Biological Timers in Vibrio harveyi
title Autoinducers Act as Biological Timers in Vibrio harveyi
title_full Autoinducers Act as Biological Timers in Vibrio harveyi
title_fullStr Autoinducers Act as Biological Timers in Vibrio harveyi
title_full_unstemmed Autoinducers Act as Biological Timers in Vibrio harveyi
title_short Autoinducers Act as Biological Timers in Vibrio harveyi
title_sort autoinducers act as biological timers in vibrio harveyi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482212/
https://www.ncbi.nlm.nih.gov/pubmed/23110227
http://dx.doi.org/10.1371/journal.pone.0048310
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