Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR

BACKGROUND: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS: We developed fluorescent amplification-based specific hybridization (FLASH–PCR) a...

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Detalles Bibliográficos
Autores principales: Khatami, F, Heidari, M, Khatami, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482334/
https://www.ncbi.nlm.nih.gov/pubmed/23115724
Descripción
Sumario:BACKGROUND: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS: We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT: PCR product of 336 bp was successfully amplified in a FLASH–PCR. CONCLUSION: As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.

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