Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR

BACKGROUND: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS: We developed fluorescent amplification-based specific hybridization (FLASH–PCR) a...

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Detalles Bibliográficos
Autores principales: Khatami, F, Heidari, M, Khatami, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482334/
https://www.ncbi.nlm.nih.gov/pubmed/23115724
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author Khatami, F
Heidari, M
Khatami, M
author_facet Khatami, F
Heidari, M
Khatami, M
author_sort Khatami, F
collection PubMed
description BACKGROUND: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS: We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT: PCR product of 336 bp was successfully amplified in a FLASH–PCR. CONCLUSION: As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.
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spelling pubmed-34823342012-10-31 Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR Khatami, F Heidari, M Khatami, M Iran Red Crescent Med J Short Communication BACKGROUND: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS: We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT: PCR product of 336 bp was successfully amplified in a FLASH–PCR. CONCLUSION: As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7. Kowsar 2012-09-30 2012-09 /pmc/articles/PMC3482334/ /pubmed/23115724 Text en Copyright © 2012, Iranian Red Crescent Medical Journal http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Khatami, F
Heidari, M
Khatami, M
Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
title Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
title_full Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
title_fullStr Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
title_full_unstemmed Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
title_short Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
title_sort rapid detection of escherichia coli o157: h7 by fluorescent amplification–based specific hybridization (flash) pcr
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482334/
https://www.ncbi.nlm.nih.gov/pubmed/23115724
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