Cargando…

Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections

INTRODUCTION: Enterococcus faecalis has long been implicated in persistent root canal infections and therapy-resistant endodontic infections. It has also been associated with bacteremia, that is, infective endocarditis arising from certain invasive dental procedures. E. faecalis endocarditis antigen...

Descripción completa

Detalles Bibliográficos
Autores principales: Preethee, Thomas, Kandaswamy, Deivanayagam, Hannah, Rosaline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482742/
https://www.ncbi.nlm.nih.gov/pubmed/23112476
http://dx.doi.org/10.4103/0972-0707.101886
_version_ 1782247901027631104
author Preethee, Thomas
Kandaswamy, Deivanayagam
Hannah, Rosaline
author_facet Preethee, Thomas
Kandaswamy, Deivanayagam
Hannah, Rosaline
author_sort Preethee, Thomas
collection PubMed
description INTRODUCTION: Enterococcus faecalis has long been implicated in persistent root canal infections and therapy-resistant endodontic infections. It has also been associated with bacteremia, that is, infective endocarditis arising from certain invasive dental procedures. E. faecalis endocarditis antigen (efaA) has been identified as one of the principal virulence factors associated with infective endocarditis. AIM: To detect the presence of putative E. faecalis virulence factor, efaA in root canals of therapy-resistant endodontic infections using polymerase chain reaction (PCR) amplification. MATERIALS AND METHODS: Samples were obtained from 32 patients (20–70 years) undergoing endodontic retreatment, which were incubated in prereduced thioglycollate broth and subcultured onto ethyl violet azide broth (EVA; selective medium for E. faecalis). Deoxyribonucleic acid (DNA) was extracted from the samples and analyzed for the endocarditis virulence factor efaA using PCR. RESULTS: Among the positive E. faecalis samples, efaA gene was identified in 11 out of 15 samples. CONCLUSION: Our findings indicate that efaA, a potent E. faecalis virulence gene can be found in E. faecalis strains detected in root canals of therapy-resistant endodontic infections similar to reports for ‘medical’ strains.
format Online
Article
Text
id pubmed-3482742
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-34827422012-10-30 Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections Preethee, Thomas Kandaswamy, Deivanayagam Hannah, Rosaline J Conserv Dent Original Article INTRODUCTION: Enterococcus faecalis has long been implicated in persistent root canal infections and therapy-resistant endodontic infections. It has also been associated with bacteremia, that is, infective endocarditis arising from certain invasive dental procedures. E. faecalis endocarditis antigen (efaA) has been identified as one of the principal virulence factors associated with infective endocarditis. AIM: To detect the presence of putative E. faecalis virulence factor, efaA in root canals of therapy-resistant endodontic infections using polymerase chain reaction (PCR) amplification. MATERIALS AND METHODS: Samples were obtained from 32 patients (20–70 years) undergoing endodontic retreatment, which were incubated in prereduced thioglycollate broth and subcultured onto ethyl violet azide broth (EVA; selective medium for E. faecalis). Deoxyribonucleic acid (DNA) was extracted from the samples and analyzed for the endocarditis virulence factor efaA using PCR. RESULTS: Among the positive E. faecalis samples, efaA gene was identified in 11 out of 15 samples. CONCLUSION: Our findings indicate that efaA, a potent E. faecalis virulence gene can be found in E. faecalis strains detected in root canals of therapy-resistant endodontic infections similar to reports for ‘medical’ strains. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3482742/ /pubmed/23112476 http://dx.doi.org/10.4103/0972-0707.101886 Text en Copyright: © Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Preethee, Thomas
Kandaswamy, Deivanayagam
Hannah, Rosaline
Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections
title Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections
title_full Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections
title_fullStr Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections
title_full_unstemmed Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections
title_short Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections
title_sort molecular identification of an enterococcus faecalis endocarditis antigen efaa in root canals of therapy-resistant endodontic infections
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482742/
https://www.ncbi.nlm.nih.gov/pubmed/23112476
http://dx.doi.org/10.4103/0972-0707.101886
work_keys_str_mv AT preetheethomas molecularidentificationofanenterococcusfaecalisendocarditisantigenefaainrootcanalsoftherapyresistantendodonticinfections
AT kandaswamydeivanayagam molecularidentificationofanenterococcusfaecalisendocarditisantigenefaainrootcanalsoftherapyresistantendodonticinfections
AT hannahrosaline molecularidentificationofanenterococcusfaecalisendocarditisantigenefaainrootcanalsoftherapyresistantendodonticinfections