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Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy

Local radiotherapy (RT) plus intratumoral dendritic cell (DC) injection can mediate immunological response. We hypothesized that co-injection of exogenous recombinant heat shock protein 70 (rHsp70) in combination with RT-DC could be as effective as co-injection of HSP-peptide for evoking specific im...

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Autores principales: Wang, Yu-Shan, Liu, Shih-Jen, Huang, Su-Chen, Chang, Chao-Chun, Huang, Yi-Chun, Fong, Weng-Lam, Chi, Mau-Shin, Chi, Kwan-Hwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483023/
https://www.ncbi.nlm.nih.gov/pubmed/23112956
http://dx.doi.org/10.3389/fonc.2012.00149
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author Wang, Yu-Shan
Liu, Shih-Jen
Huang, Su-Chen
Chang, Chao-Chun
Huang, Yi-Chun
Fong, Weng-Lam
Chi, Mau-Shin
Chi, Kwan-Hwa
author_facet Wang, Yu-Shan
Liu, Shih-Jen
Huang, Su-Chen
Chang, Chao-Chun
Huang, Yi-Chun
Fong, Weng-Lam
Chi, Mau-Shin
Chi, Kwan-Hwa
author_sort Wang, Yu-Shan
collection PubMed
description Local radiotherapy (RT) plus intratumoral dendritic cell (DC) injection can mediate immunological response. We hypothesized that co-injection of exogenous recombinant heat shock protein 70 (rHsp70) in combination with RT-DC could be as effective as co-injection of HSP-peptide for evoking specific immune response. rHsp70-prostate-specific antigen (rHSP70C′-PSA) and α-fetoprotein (rHSP70C′-AFP) were used to compare specific response. Growth inhibition of the tumor and the systemic anti-tumor immune response were measured on CT26/PSA and CT26/AFP mice model. Intratumoral co-injection of rHsp70 and DC into the irradiated tumor site induced a more potent anti-tumor immune response than injection of DC alone. rHsp70 was as effective as rHsp70C′-PSA or rHsp70C′-AFP in inducing a tumor-specific cytotoxic T lymphocyte response or tumor growth delay. These results demonstrate that co-administration with rHsp70 and RT could be a simple and effective source of tumor antigens to achieve RT-DC immunotherapy protocol and easy to apply in clinical use.
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spelling pubmed-34830232012-10-30 Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy Wang, Yu-Shan Liu, Shih-Jen Huang, Su-Chen Chang, Chao-Chun Huang, Yi-Chun Fong, Weng-Lam Chi, Mau-Shin Chi, Kwan-Hwa Front Oncol Oncology Local radiotherapy (RT) plus intratumoral dendritic cell (DC) injection can mediate immunological response. We hypothesized that co-injection of exogenous recombinant heat shock protein 70 (rHsp70) in combination with RT-DC could be as effective as co-injection of HSP-peptide for evoking specific immune response. rHsp70-prostate-specific antigen (rHSP70C′-PSA) and α-fetoprotein (rHSP70C′-AFP) were used to compare specific response. Growth inhibition of the tumor and the systemic anti-tumor immune response were measured on CT26/PSA and CT26/AFP mice model. Intratumoral co-injection of rHsp70 and DC into the irradiated tumor site induced a more potent anti-tumor immune response than injection of DC alone. rHsp70 was as effective as rHsp70C′-PSA or rHsp70C′-AFP in inducing a tumor-specific cytotoxic T lymphocyte response or tumor growth delay. These results demonstrate that co-administration with rHsp70 and RT could be a simple and effective source of tumor antigens to achieve RT-DC immunotherapy protocol and easy to apply in clinical use. Frontiers Media S.A. 2012-10-29 /pmc/articles/PMC3483023/ /pubmed/23112956 http://dx.doi.org/10.3389/fonc.2012.00149 Text en Copyright © 2012 Wang, Liu, Huang, Chang, Huang, Fong, Chi and Chi. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Oncology
Wang, Yu-Shan
Liu, Shih-Jen
Huang, Su-Chen
Chang, Chao-Chun
Huang, Yi-Chun
Fong, Weng-Lam
Chi, Mau-Shin
Chi, Kwan-Hwa
Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
title Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
title_full Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
title_fullStr Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
title_full_unstemmed Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
title_short Recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
title_sort recombinant heat shock protein 70 in combination with radiotherapy as a source of tumor antigens to improve dendritic cell immunotherapy
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483023/
https://www.ncbi.nlm.nih.gov/pubmed/23112956
http://dx.doi.org/10.3389/fonc.2012.00149
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