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NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries

The identification of active ingredients in crude plant extracts offers great advantages. In this study, nuclear magnetic resonance and chemometrics were used for the screening of in vitro anti-TNFα activity in different berry types. Solid phase extraction was applied and the resulting water, methan...

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Autores principales: Ali, Kashif, Iqbal, Muzamal, Korthout, Henrie A. A. J., Maltese, Federica, Fortes, Ana Margarida, Pais, Maria Salomé, Verpoorte, Robert, Choi, Young Hae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483097/
https://www.ncbi.nlm.nih.gov/pubmed/23136560
http://dx.doi.org/10.1007/s11306-012-0406-8
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author Ali, Kashif
Iqbal, Muzamal
Korthout, Henrie A. A. J.
Maltese, Federica
Fortes, Ana Margarida
Pais, Maria Salomé
Verpoorte, Robert
Choi, Young Hae
author_facet Ali, Kashif
Iqbal, Muzamal
Korthout, Henrie A. A. J.
Maltese, Federica
Fortes, Ana Margarida
Pais, Maria Salomé
Verpoorte, Robert
Choi, Young Hae
author_sort Ali, Kashif
collection PubMed
description The identification of active ingredients in crude plant extracts offers great advantages. In this study, nuclear magnetic resonance and chemometrics were used for the screening of in vitro anti-TNFα activity in different berry types. Solid phase extraction was applied and the resulting water, methanol–water (1:1), and methanol fractions were tested for the activity. The methanol–water fraction contained most of the phenolics and showed significantly higher activity than the other two fractions. In the second phase of this study, grapes from ‘Trincadeira’, ‘Touriga Nacional’, and ‘Aragonês’, at four developmental stages were metabolically classified and tested for the TNFα inhibition. The initial stages of grape development, green and veraison, were found more active against TNFα production as compared to the later ripe and harvest stages. Among the cultivars, ‘Touriga Nacional’ was found to be the most potent inhibitor. Different multivariate data analyses algorithms based on projections to latent structures were applied to correlate the NMR and TNFα inhibition data. The variable importance in the projections plot showed that phenolics like quercetin, myricetin, (+)-catechin, (−)-epicatechin, caftarate, and coutarate, were positively correlated with high activity. This work demonstrates the great potential of NMR spectroscopy in combination with chemometrics for the screening of large set of crude extracts, to study the effects of different variables on the activity, and identifying active compounds in complex mixtures like plant extracts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-012-0406-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-34830972012-11-05 NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries Ali, Kashif Iqbal, Muzamal Korthout, Henrie A. A. J. Maltese, Federica Fortes, Ana Margarida Pais, Maria Salomé Verpoorte, Robert Choi, Young Hae Metabolomics Original Article The identification of active ingredients in crude plant extracts offers great advantages. In this study, nuclear magnetic resonance and chemometrics were used for the screening of in vitro anti-TNFα activity in different berry types. Solid phase extraction was applied and the resulting water, methanol–water (1:1), and methanol fractions were tested for the activity. The methanol–water fraction contained most of the phenolics and showed significantly higher activity than the other two fractions. In the second phase of this study, grapes from ‘Trincadeira’, ‘Touriga Nacional’, and ‘Aragonês’, at four developmental stages were metabolically classified and tested for the TNFα inhibition. The initial stages of grape development, green and veraison, were found more active against TNFα production as compared to the later ripe and harvest stages. Among the cultivars, ‘Touriga Nacional’ was found to be the most potent inhibitor. Different multivariate data analyses algorithms based on projections to latent structures were applied to correlate the NMR and TNFα inhibition data. The variable importance in the projections plot showed that phenolics like quercetin, myricetin, (+)-catechin, (−)-epicatechin, caftarate, and coutarate, were positively correlated with high activity. This work demonstrates the great potential of NMR spectroscopy in combination with chemometrics for the screening of large set of crude extracts, to study the effects of different variables on the activity, and identifying active compounds in complex mixtures like plant extracts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-012-0406-8) contains supplementary material, which is available to authorized users. Springer US 2012-02-17 2012 /pmc/articles/PMC3483097/ /pubmed/23136560 http://dx.doi.org/10.1007/s11306-012-0406-8 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Ali, Kashif
Iqbal, Muzamal
Korthout, Henrie A. A. J.
Maltese, Federica
Fortes, Ana Margarida
Pais, Maria Salomé
Verpoorte, Robert
Choi, Young Hae
NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries
title NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries
title_full NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries
title_fullStr NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries
title_full_unstemmed NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries
title_short NMR spectroscopy and chemometrics as a tool for anti-TNFα activity screening in crude extracts of grapes and other berries
title_sort nmr spectroscopy and chemometrics as a tool for anti-tnfα activity screening in crude extracts of grapes and other berries
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483097/
https://www.ncbi.nlm.nih.gov/pubmed/23136560
http://dx.doi.org/10.1007/s11306-012-0406-8
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