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Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling
Cranial neural crest cells form much of the facial skeleton, and abnormalities in their development lead to severe birth defects. In a novel zebrafish protein trap screen, we identified an RNA-binding protein, Rbms3, that is transiently expressed in the cytoplasm of condensing neural crest cells wit...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483135/ https://www.ncbi.nlm.nih.gov/pubmed/23091072 http://dx.doi.org/10.1083/jcb.201204138 |
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author | Jayasena, Chathurani S. Bronner, Marianne E. |
author_facet | Jayasena, Chathurani S. Bronner, Marianne E. |
author_sort | Jayasena, Chathurani S. |
collection | PubMed |
description | Cranial neural crest cells form much of the facial skeleton, and abnormalities in their development lead to severe birth defects. In a novel zebrafish protein trap screen, we identified an RNA-binding protein, Rbms3, that is transiently expressed in the cytoplasm of condensing neural crest cells within the pharyngeal arches. Morphants for rbms3 displayed reduced proliferation of prechondrogenic crest and significantly altered expression for chondrogenic/osteogenic lineage markers. This phenotype strongly resembles cartilage/crest defects observed in Tgf-βr2:Wnt1-Cre mutants, which suggests a possible link with TGF-β signaling. Consistent with this are the findings that: (a) Rbms3 stabilized a reporter transcript with smad2 3′ untranslated region, (b) RNA immunoprecipitation with full-length Rbms3 showed enrichment for smad2/3, and (c) pSmad2 levels were reduced in rbms3 morphants. Overall, these results suggest that Rbms3 posttranscriptionally regulates one of the major pathways that promotes chondrogenesis, the transforming growth factor β receptor (TGF-βr) pathway. |
format | Online Article Text |
id | pubmed-3483135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34831352013-04-29 Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling Jayasena, Chathurani S. Bronner, Marianne E. J Cell Biol Research Articles Cranial neural crest cells form much of the facial skeleton, and abnormalities in their development lead to severe birth defects. In a novel zebrafish protein trap screen, we identified an RNA-binding protein, Rbms3, that is transiently expressed in the cytoplasm of condensing neural crest cells within the pharyngeal arches. Morphants for rbms3 displayed reduced proliferation of prechondrogenic crest and significantly altered expression for chondrogenic/osteogenic lineage markers. This phenotype strongly resembles cartilage/crest defects observed in Tgf-βr2:Wnt1-Cre mutants, which suggests a possible link with TGF-β signaling. Consistent with this are the findings that: (a) Rbms3 stabilized a reporter transcript with smad2 3′ untranslated region, (b) RNA immunoprecipitation with full-length Rbms3 showed enrichment for smad2/3, and (c) pSmad2 levels were reduced in rbms3 morphants. Overall, these results suggest that Rbms3 posttranscriptionally regulates one of the major pathways that promotes chondrogenesis, the transforming growth factor β receptor (TGF-βr) pathway. The Rockefeller University Press 2012-10-29 /pmc/articles/PMC3483135/ /pubmed/23091072 http://dx.doi.org/10.1083/jcb.201204138 Text en © 2012 Jayasena and Bronner This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Jayasena, Chathurani S. Bronner, Marianne E. Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling |
title | Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling |
title_full | Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling |
title_fullStr | Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling |
title_full_unstemmed | Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling |
title_short | Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-β signaling |
title_sort | rbms3 functions in craniofacial development by posttranscriptionally modulating tgf-β signaling |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483135/ https://www.ncbi.nlm.nih.gov/pubmed/23091072 http://dx.doi.org/10.1083/jcb.201204138 |
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