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Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice

Antimicrobial peptides have been suggested as an alternative to classical antibiotics in livestock production and bacteriocin-producing bacteria could be added to animal feeds to deliver bacteriocins in the gastrointestinal (GI) tract of ruminant and monogastric animals. In this study, viable (V) an...

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Autores principales: Paiva, Aline D., Fernandes, Kenner M., Dias, Roberto S., Rocha, Alípio S., de Oliveira, Leandro L., Neves, Clóvis A., de Paula, Sérgio O., Mantovani, Hilário C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483269/
https://www.ncbi.nlm.nih.gov/pubmed/23144752
http://dx.doi.org/10.1371/journal.pone.0048313
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author Paiva, Aline D.
Fernandes, Kenner M.
Dias, Roberto S.
Rocha, Alípio S.
de Oliveira, Leandro L.
Neves, Clóvis A.
de Paula, Sérgio O.
Mantovani, Hilário C.
author_facet Paiva, Aline D.
Fernandes, Kenner M.
Dias, Roberto S.
Rocha, Alípio S.
de Oliveira, Leandro L.
Neves, Clóvis A.
de Paula, Sérgio O.
Mantovani, Hilário C.
author_sort Paiva, Aline D.
collection PubMed
description Antimicrobial peptides have been suggested as an alternative to classical antibiotics in livestock production and bacteriocin-producing bacteria could be added to animal feeds to deliver bacteriocins in the gastrointestinal (GI) tract of ruminant and monogastric animals. In this study, viable (V) and heat-killed (HK) Streptococcus bovis HC5 cells were orally administered to pre-sensitized mice in order to assess the effects of a bacteriocin-producing bacteria on histological parameters and the immune response of the GI tract of monogastric animals. The administration of V and HK S. bovis HC5 cells during 58 days to BALB/c mice did not affect weight gain, but an increase in gut permeability was detected in animals receiving the HK cells. Viable and heat killed cells caused similar morphological alterations in the GI tract of the animals, but the most prominent effects were detected in the small intestine. The oral administration of S. bovis HC5 also influenced cytokine production in the small intestine, and the immune-mediated activity differed between V and HK cells. The relative expression of IL-12 and INF-γ was significantly higher in the small intestine of mice treated with V cells, while an increase in IL-5, IL-13 and TNF-α expression was only detected in mice treated with HK cells. Considering that even under a condition of severe challenge (pre-sensitization followed by daily exposure to the same bacterial immunogen) the general health of the animals was maintained, it appears that oral administration of S. bovis HC5 cells could be a useful route to deliver bacteriocin in the GI tract of livestock animals.
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spelling pubmed-34832692012-11-09 Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice Paiva, Aline D. Fernandes, Kenner M. Dias, Roberto S. Rocha, Alípio S. de Oliveira, Leandro L. Neves, Clóvis A. de Paula, Sérgio O. Mantovani, Hilário C. PLoS One Research Article Antimicrobial peptides have been suggested as an alternative to classical antibiotics in livestock production and bacteriocin-producing bacteria could be added to animal feeds to deliver bacteriocins in the gastrointestinal (GI) tract of ruminant and monogastric animals. In this study, viable (V) and heat-killed (HK) Streptococcus bovis HC5 cells were orally administered to pre-sensitized mice in order to assess the effects of a bacteriocin-producing bacteria on histological parameters and the immune response of the GI tract of monogastric animals. The administration of V and HK S. bovis HC5 cells during 58 days to BALB/c mice did not affect weight gain, but an increase in gut permeability was detected in animals receiving the HK cells. Viable and heat killed cells caused similar morphological alterations in the GI tract of the animals, but the most prominent effects were detected in the small intestine. The oral administration of S. bovis HC5 also influenced cytokine production in the small intestine, and the immune-mediated activity differed between V and HK cells. The relative expression of IL-12 and INF-γ was significantly higher in the small intestine of mice treated with V cells, while an increase in IL-5, IL-13 and TNF-α expression was only detected in mice treated with HK cells. Considering that even under a condition of severe challenge (pre-sensitization followed by daily exposure to the same bacterial immunogen) the general health of the animals was maintained, it appears that oral administration of S. bovis HC5 cells could be a useful route to deliver bacteriocin in the GI tract of livestock animals. Public Library of Science 2012-10-29 /pmc/articles/PMC3483269/ /pubmed/23144752 http://dx.doi.org/10.1371/journal.pone.0048313 Text en © 2012 Paiva et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Paiva, Aline D.
Fernandes, Kenner M.
Dias, Roberto S.
Rocha, Alípio S.
de Oliveira, Leandro L.
Neves, Clóvis A.
de Paula, Sérgio O.
Mantovani, Hilário C.
Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice
title Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice
title_full Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice
title_fullStr Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice
title_full_unstemmed Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice
title_short Effects of the Oral Administration of Viable and Heat-Killed Streptococcus bovis HC5 Cells to Pre-Sensitized BALB/c Mice
title_sort effects of the oral administration of viable and heat-killed streptococcus bovis hc5 cells to pre-sensitized balb/c mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483269/
https://www.ncbi.nlm.nih.gov/pubmed/23144752
http://dx.doi.org/10.1371/journal.pone.0048313
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