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BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses

The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized h...

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Detalles Bibliográficos
Autores principales: De Smet, Lina, Ravoet, Jorgen, de Miranda, Joachim R., Wenseleers, Tom, Mueller, Matthias Y., Moritz, Robin F. A., de Graaf, Dirk C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483297/
https://www.ncbi.nlm.nih.gov/pubmed/23144717
http://dx.doi.org/10.1371/journal.pone.0047953
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author De Smet, Lina
Ravoet, Jorgen
de Miranda, Joachim R.
Wenseleers, Tom
Mueller, Matthias Y.
Moritz, Robin F. A.
de Graaf, Dirk C.
author_facet De Smet, Lina
Ravoet, Jorgen
de Miranda, Joachim R.
Wenseleers, Tom
Mueller, Matthias Y.
Moritz, Robin F. A.
de Graaf, Dirk C.
author_sort De Smet, Lina
collection PubMed
description The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized honeybee viruses (around twenty) are positive stranded RNA viruses. Techniques based on RNA signatures are widely used to determine the viral load in honeybee colonies. High throughput screening for viral loads necessitates the development of a multiplex polymerase chain reaction approach in which different viruses can be targeted simultaneously. A new multiparameter assay, called “BeeDoctor”, was developed based on multiplex-ligation probe dependent amplification (MLPA) technology. This assay detects 10 honeybee viruses in one reaction. “BeeDoctor” is also able to screen selectively for either the positive strand of the targeted RNA bee viruses or the negative strand, which is indicative for active viral replication. Due to its sensitivity and specificity, the MLPA assay is a useful tool for rapid diagnosis, pathogen characterization, and epidemiology of viruses in honeybee populations. “BeeDoctor” was used for screening 363 samples from apiaries located throughout Flanders; the northern half of Belgium. Using the “BeeDoctor”, virus infections were detected in almost eighty percent of the colonies, with deformed wing virus by far the most frequently detected virus and multiple virus infections were found in 26 percent of the colonies.
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spelling pubmed-34832972012-11-09 BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses De Smet, Lina Ravoet, Jorgen de Miranda, Joachim R. Wenseleers, Tom Mueller, Matthias Y. Moritz, Robin F. A. de Graaf, Dirk C. PLoS One Research Article The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized honeybee viruses (around twenty) are positive stranded RNA viruses. Techniques based on RNA signatures are widely used to determine the viral load in honeybee colonies. High throughput screening for viral loads necessitates the development of a multiplex polymerase chain reaction approach in which different viruses can be targeted simultaneously. A new multiparameter assay, called “BeeDoctor”, was developed based on multiplex-ligation probe dependent amplification (MLPA) technology. This assay detects 10 honeybee viruses in one reaction. “BeeDoctor” is also able to screen selectively for either the positive strand of the targeted RNA bee viruses or the negative strand, which is indicative for active viral replication. Due to its sensitivity and specificity, the MLPA assay is a useful tool for rapid diagnosis, pathogen characterization, and epidemiology of viruses in honeybee populations. “BeeDoctor” was used for screening 363 samples from apiaries located throughout Flanders; the northern half of Belgium. Using the “BeeDoctor”, virus infections were detected in almost eighty percent of the colonies, with deformed wing virus by far the most frequently detected virus and multiple virus infections were found in 26 percent of the colonies. Public Library of Science 2012-10-29 /pmc/articles/PMC3483297/ /pubmed/23144717 http://dx.doi.org/10.1371/journal.pone.0047953 Text en © 2012 De Smet et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
De Smet, Lina
Ravoet, Jorgen
de Miranda, Joachim R.
Wenseleers, Tom
Mueller, Matthias Y.
Moritz, Robin F. A.
de Graaf, Dirk C.
BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
title BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
title_full BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
title_fullStr BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
title_full_unstemmed BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
title_short BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
title_sort beedoctor, a versatile mlpa-based diagnostic tool for screening bee viruses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483297/
https://www.ncbi.nlm.nih.gov/pubmed/23144717
http://dx.doi.org/10.1371/journal.pone.0047953
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