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Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study
BACKGROUND: The activation of guanine nucleotide binding protein-coupled receptors, such as adenosine receptor (ADR) and opioid receptor (OPR), protects the heart against ischemia and reperfusion injury. We hypothesized that ADR or OPR might be involved in polyphenol (-)-epigallocatechin gallate (EG...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Anesthesiologists
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483493/ https://www.ncbi.nlm.nih.gov/pubmed/23115687 http://dx.doi.org/10.4097/kjae.2012.63.4.340 |
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author | Lee, Sang Kwon Kim, June Hong Kim, Jeong Su Jang, Youngho Kim, Jun Park, Yong Hyun Chun, Kook Jin Lee, Mi Young |
author_facet | Lee, Sang Kwon Kim, June Hong Kim, Jeong Su Jang, Youngho Kim, Jun Park, Yong Hyun Chun, Kook Jin Lee, Mi Young |
author_sort | Lee, Sang Kwon |
collection | PubMed |
description | BACKGROUND: The activation of guanine nucleotide binding protein-coupled receptors, such as adenosine receptor (ADR) and opioid receptor (OPR), protects the heart against ischemia and reperfusion injury. We hypothesized that ADR or OPR might be involved in polyphenol (-)-epigallocatechin gallate (EGCG)-induced cardioprotection. METHODS: Langendorff perfused rat hearts were subjected to 30 min of regional ischemia and 2 h of reperfusion. Hearts were treated with 10 µM of EGCG, with or without the ADR or OPR antagonist at early reperfusion. Infarct size measured with 2,3,5-triphenyltetrazolium chloride staining was chosen as end-point. RESULTS: EGCG significantly reduced infarct volume as a percentage of ischemic volume (33.5 ± 4.1%) compared to control hearts (14.4 ± 1.1%, P < 0.001). A nonspecific ADR antagonist 8-(p-sulfophenyl) theophylline hydrate (27.1 ± 1.9%, P < 0.05 vs. EGCG) but not a nonspecific OPR antagonist naloxone (14.3 ± 1.3%, P > 0.05 vs. EGCG) blocked the anti-infarct effect by EGCG. The infarct reducing effect of EGCG was significantly reversed by 200 nM of the A(1) ADR antagonist DPCPX (25.9 ± 1.1%, P < 0.05) and 15 nM of the A(2B) ADR antagonist MRS1706 (29.3 ± 1.7%, P < 0.01) but not by 10 µM of the A(2A) ADR antagonist ZM241385 (23.9 ± 1.9%. P > 0.05 vs. EGCG) and 100 nM of the A(3) ADR antagonist MRS1334 (24.1 ± 1.8%, P > 0.05). CONCLUSIONS: The infarct reducing effect of EGCG appears to involve activation of ADR, especially A(1) and A(2B) ADR, but not OPR. |
format | Online Article Text |
id | pubmed-3483493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Society of Anesthesiologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-34834932012-10-31 Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study Lee, Sang Kwon Kim, June Hong Kim, Jeong Su Jang, Youngho Kim, Jun Park, Yong Hyun Chun, Kook Jin Lee, Mi Young Korean J Anesthesiol Experimental Research Article BACKGROUND: The activation of guanine nucleotide binding protein-coupled receptors, such as adenosine receptor (ADR) and opioid receptor (OPR), protects the heart against ischemia and reperfusion injury. We hypothesized that ADR or OPR might be involved in polyphenol (-)-epigallocatechin gallate (EGCG)-induced cardioprotection. METHODS: Langendorff perfused rat hearts were subjected to 30 min of regional ischemia and 2 h of reperfusion. Hearts were treated with 10 µM of EGCG, with or without the ADR or OPR antagonist at early reperfusion. Infarct size measured with 2,3,5-triphenyltetrazolium chloride staining was chosen as end-point. RESULTS: EGCG significantly reduced infarct volume as a percentage of ischemic volume (33.5 ± 4.1%) compared to control hearts (14.4 ± 1.1%, P < 0.001). A nonspecific ADR antagonist 8-(p-sulfophenyl) theophylline hydrate (27.1 ± 1.9%, P < 0.05 vs. EGCG) but not a nonspecific OPR antagonist naloxone (14.3 ± 1.3%, P > 0.05 vs. EGCG) blocked the anti-infarct effect by EGCG. The infarct reducing effect of EGCG was significantly reversed by 200 nM of the A(1) ADR antagonist DPCPX (25.9 ± 1.1%, P < 0.05) and 15 nM of the A(2B) ADR antagonist MRS1706 (29.3 ± 1.7%, P < 0.01) but not by 10 µM of the A(2A) ADR antagonist ZM241385 (23.9 ± 1.9%. P > 0.05 vs. EGCG) and 100 nM of the A(3) ADR antagonist MRS1334 (24.1 ± 1.8%, P > 0.05). CONCLUSIONS: The infarct reducing effect of EGCG appears to involve activation of ADR, especially A(1) and A(2B) ADR, but not OPR. The Korean Society of Anesthesiologists 2012-10 2012-10-12 /pmc/articles/PMC3483493/ /pubmed/23115687 http://dx.doi.org/10.4097/kjae.2012.63.4.340 Text en Copyright © the Korean Society of Anesthesiologists, 2012 http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Experimental Research Article Lee, Sang Kwon Kim, June Hong Kim, Jeong Su Jang, Youngho Kim, Jun Park, Yong Hyun Chun, Kook Jin Lee, Mi Young Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
title | Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
title_full | Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
title_fullStr | Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
title_full_unstemmed | Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
title_short | Polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
title_sort | polyphenol (-)-epigallocatechin gallate-induced cardioprotection may attenuate ischemia-reperfusion injury through adenosine receptor activation: a preliminary study |
topic | Experimental Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483493/ https://www.ncbi.nlm.nih.gov/pubmed/23115687 http://dx.doi.org/10.4097/kjae.2012.63.4.340 |
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