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Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)
Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), c...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific World Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483669/ https://www.ncbi.nlm.nih.gov/pubmed/23125558 http://dx.doi.org/10.1100/2012/859381 |
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author | Chae, Soo Cheon Kim, Haeng Hoon Park, Sang Un |
author_facet | Chae, Soo Cheon Kim, Haeng Hoon Park, Sang Un |
author_sort | Chae, Soo Cheon |
collection | PubMed |
description | Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl(2)), and silver thiosulphate (STS). The addition of AVG, CoCl(2), and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl(2) the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl(2) at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol in S. speciosa for plant regenerations. |
format | Online Article Text |
id | pubmed-3483669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Scientific World Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-34836692012-11-02 Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) Chae, Soo Cheon Kim, Haeng Hoon Park, Sang Un ScientificWorldJournal Research Article Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl(2)), and silver thiosulphate (STS). The addition of AVG, CoCl(2), and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl(2) the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl(2) at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol in S. speciosa for plant regenerations. The Scientific World Journal 2012-10-17 /pmc/articles/PMC3483669/ /pubmed/23125558 http://dx.doi.org/10.1100/2012/859381 Text en Copyright © 2012 Soo Cheon Chae et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chae, Soo Cheon Kim, Haeng Hoon Park, Sang Un Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) |
title | Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) |
title_full | Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) |
title_fullStr | Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) |
title_full_unstemmed | Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) |
title_short | Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa) |
title_sort | ethylene inhibitors enhance shoot organogenesis of gloxinia (sinningia speciosa) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483669/ https://www.ncbi.nlm.nih.gov/pubmed/23125558 http://dx.doi.org/10.1100/2012/859381 |
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