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cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus

Gene expression of neuronal nitric oxide synthase (nNOS) changes in the hypothalamic paraventricular nucleus (PVN) depending on feeding conditions, which is decreased during food deprivation and restored by refeeding, and phosphorylated cAMP response element binding protein (pCREB) was suggested to...

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Autores principales: Yoo, Sang Bae, Lee, Seoul, Lee, Joo Young, Kim, Bom-Taeck, Lee, Jong-Ho, Jahng, Jeong Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Physiological Society and The Korean Society of Pharmacology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484518/
https://www.ncbi.nlm.nih.gov/pubmed/23118557
http://dx.doi.org/10.4196/kjpp.2012.16.5.333
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author Yoo, Sang Bae
Lee, Seoul
Lee, Joo Young
Kim, Bom-Taeck
Lee, Jong-Ho
Jahng, Jeong Won
author_facet Yoo, Sang Bae
Lee, Seoul
Lee, Joo Young
Kim, Bom-Taeck
Lee, Jong-Ho
Jahng, Jeong Won
author_sort Yoo, Sang Bae
collection PubMed
description Gene expression of neuronal nitric oxide synthase (nNOS) changes in the hypothalamic paraventricular nucleus (PVN) depending on feeding conditions, which is decreased during food deprivation and restored by refeeding, and phosphorylated cAMP response element binding protein (pCREB) was suggested to play a role in its regulation. This study was conducted to examine if the fasting-induced down-regulation of the PVN-nNOS expression is restored by activation of cAMP-dependent protein kinase A (cAMP/PKA) pathway. Freely moving rats received intracerebroventricular (icv) injection of cAMP/PKA activator Sp-cAMP (40 nmol) or vehicle (sterilized saline) following 48 h of food deprivation. One hour after drug injections, rats were transcardially perfused with 4% paraformaldehyde, and the PVN tissues were processed for nNOS or pCREB immunohistochemistry. Sp-cAMP significantly increased not only nNOS but also pCREB immunoreactivities in the PVN of food deprived rats. Fasting-induced down-regulation of the PVN-nNOS was restored by 1 h after the icv Sp-cAMP. Results suggest that cAMP/PKA pathway may mediate the regulation of the PVN-nNOS expression depending on different feeding conditions.
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spelling pubmed-34845182012-11-01 cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus Yoo, Sang Bae Lee, Seoul Lee, Joo Young Kim, Bom-Taeck Lee, Jong-Ho Jahng, Jeong Won Korean J Physiol Pharmacol Original Article Gene expression of neuronal nitric oxide synthase (nNOS) changes in the hypothalamic paraventricular nucleus (PVN) depending on feeding conditions, which is decreased during food deprivation and restored by refeeding, and phosphorylated cAMP response element binding protein (pCREB) was suggested to play a role in its regulation. This study was conducted to examine if the fasting-induced down-regulation of the PVN-nNOS expression is restored by activation of cAMP-dependent protein kinase A (cAMP/PKA) pathway. Freely moving rats received intracerebroventricular (icv) injection of cAMP/PKA activator Sp-cAMP (40 nmol) or vehicle (sterilized saline) following 48 h of food deprivation. One hour after drug injections, rats were transcardially perfused with 4% paraformaldehyde, and the PVN tissues were processed for nNOS or pCREB immunohistochemistry. Sp-cAMP significantly increased not only nNOS but also pCREB immunoreactivities in the PVN of food deprived rats. Fasting-induced down-regulation of the PVN-nNOS was restored by 1 h after the icv Sp-cAMP. Results suggest that cAMP/PKA pathway may mediate the regulation of the PVN-nNOS expression depending on different feeding conditions. The Korean Physiological Society and The Korean Society of Pharmacology 2012-10 2012-10-18 /pmc/articles/PMC3484518/ /pubmed/23118557 http://dx.doi.org/10.4196/kjpp.2012.16.5.333 Text en Copyright © 2012 The Korean Physiological Society and The Korean Society of Pharmacology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoo, Sang Bae
Lee, Seoul
Lee, Joo Young
Kim, Bom-Taeck
Lee, Jong-Ho
Jahng, Jeong Won
cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
title cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
title_full cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
title_fullStr cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
title_full_unstemmed cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
title_short cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus
title_sort camp/pka agonist restores the fasting-induced down-regulation of nnos expression in the paraventricular nucleus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484518/
https://www.ncbi.nlm.nih.gov/pubmed/23118557
http://dx.doi.org/10.4196/kjpp.2012.16.5.333
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