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A Window into Domain Amplification Through Piccolo in Teleost Fish

I describe and characterize the extensive amplification of the zinc finger domain of Piccolo selectively in teleost fish. Piccolo and Bassoon are partially functionally redundant and play roles in regulating the pool of neurotransmitter-filled synaptic vesicles present at synapses. In mice, each pro...

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Autor principal: Nonet, Michael L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484663/
https://www.ncbi.nlm.nih.gov/pubmed/23173084
http://dx.doi.org/10.1534/g3.112.003624
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author Nonet, Michael L.
author_facet Nonet, Michael L.
author_sort Nonet, Michael L.
collection PubMed
description I describe and characterize the extensive amplification of the zinc finger domain of Piccolo selectively in teleost fish. Piccolo and Bassoon are partially functionally redundant and play roles in regulating the pool of neurotransmitter-filled synaptic vesicles present at synapses. In mice, each protein contains two N-terminal zinc finger domains that have been implicated in interacting with synaptic vesicles. In all teleosts examined, both the Bassoon and Piccolo genes are duplicated. Both teleost bassoon genes and one piccolo gene show very similar domain structure and intron-exon organization to their mouse homologs. In contrast, in piccolo b a single exon that encodes a zinc finger domain is amplified 8 to 16 times in different teleost species. Analysis of the amplified exons suggests they were added and/or deleted from the gene as individual exons in rare events that are likely the result of unequal crossovers between homologous sequences. Surprisingly, the structure of the repeats from cod and zebrafish suggest that amplification of this exon has occurred independently multiple times in the teleost lineage. Based on the structure of the exons, I propose a model in which selection for high sequence similarity at the 5′ and 3′ ends of the exon drives amplification of the repeats and diversity in repeat length likely promotes the stability of the repeated exons by minimizing the likelihood of mispairing of adjacent repeat sequences. Further analysis of piccolo b in teleosts should provide a window through which to examine the process of domain amplification.
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spelling pubmed-34846632012-11-21 A Window into Domain Amplification Through Piccolo in Teleost Fish Nonet, Michael L. G3 (Bethesda) Investigations I describe and characterize the extensive amplification of the zinc finger domain of Piccolo selectively in teleost fish. Piccolo and Bassoon are partially functionally redundant and play roles in regulating the pool of neurotransmitter-filled synaptic vesicles present at synapses. In mice, each protein contains two N-terminal zinc finger domains that have been implicated in interacting with synaptic vesicles. In all teleosts examined, both the Bassoon and Piccolo genes are duplicated. Both teleost bassoon genes and one piccolo gene show very similar domain structure and intron-exon organization to their mouse homologs. In contrast, in piccolo b a single exon that encodes a zinc finger domain is amplified 8 to 16 times in different teleost species. Analysis of the amplified exons suggests they were added and/or deleted from the gene as individual exons in rare events that are likely the result of unequal crossovers between homologous sequences. Surprisingly, the structure of the repeats from cod and zebrafish suggest that amplification of this exon has occurred independently multiple times in the teleost lineage. Based on the structure of the exons, I propose a model in which selection for high sequence similarity at the 5′ and 3′ ends of the exon drives amplification of the repeats and diversity in repeat length likely promotes the stability of the repeated exons by minimizing the likelihood of mispairing of adjacent repeat sequences. Further analysis of piccolo b in teleosts should provide a window through which to examine the process of domain amplification. Genetics Society of America 2012-11-01 /pmc/articles/PMC3484663/ /pubmed/23173084 http://dx.doi.org/10.1534/g3.112.003624 Text en Copyright © 2012 Nonet http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Nonet, Michael L.
A Window into Domain Amplification Through Piccolo in Teleost Fish
title A Window into Domain Amplification Through Piccolo in Teleost Fish
title_full A Window into Domain Amplification Through Piccolo in Teleost Fish
title_fullStr A Window into Domain Amplification Through Piccolo in Teleost Fish
title_full_unstemmed A Window into Domain Amplification Through Piccolo in Teleost Fish
title_short A Window into Domain Amplification Through Piccolo in Teleost Fish
title_sort window into domain amplification through piccolo in teleost fish
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484663/
https://www.ncbi.nlm.nih.gov/pubmed/23173084
http://dx.doi.org/10.1534/g3.112.003624
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