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Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells

BACKGROUND: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease...

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Autores principales: Shi, Si-Feng, Jia, Jing-Fu, Guo, Xiao-Kui, Zhao, Ya-Ping, Chen, De-Sheng, Guo, Yong-Yuan, Cheng, Tao, Zhang, Xian-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484720/
https://www.ncbi.nlm.nih.gov/pubmed/23118539
http://dx.doi.org/10.2147/IJN.S34348
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author Shi, Si-Feng
Jia, Jing-Fu
Guo, Xiao-Kui
Zhao, Ya-Ping
Chen, De-Sheng
Guo, Yong-Yuan
Cheng, Tao
Zhang, Xian-Long
author_facet Shi, Si-Feng
Jia, Jing-Fu
Guo, Xiao-Kui
Zhao, Ya-Ping
Chen, De-Sheng
Guo, Yong-Yuan
Cheng, Tao
Zhang, Xian-Long
author_sort Shi, Si-Feng
collection PubMed
description BACKGROUND: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease. Chitosan is widely used in the biomedical community, including for orthopedic applications. The aim of the present study was to coat chitosan onto iron oxide nanoparticles and to determine its effect on the proliferation and differentiation of osteoblasts. METHODS: Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, and vibrating sample magnetometry. Uptake of nanoparticles by osteoblasts was studied by transmission electron microscopy and Prussian blue staining. Viability and proliferation of osteoblasts were measured in the presence of uncoated iron oxide magnetic nanoparticles or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, and extracellular calcium deposition was studied in the presence of the nanoparticles. RESULTS: Chitosan-coated iron oxide nanoparticles enhanced osteoblast proliferation, decreased cell membrane damage, and promoted cell differentiation, as indicated by an increase in alkaline phosphatase and extracellular calcium deposition. Chitosan-coated iron oxide nanoparticles showed good compatibility with osteoblasts. CONCLUSION: Further research is necessary to optimize magnetic nanoparticles for the treatment of bone disease.
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spelling pubmed-34847202012-11-01 Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells Shi, Si-Feng Jia, Jing-Fu Guo, Xiao-Kui Zhao, Ya-Ping Chen, De-Sheng Guo, Yong-Yuan Cheng, Tao Zhang, Xian-Long Int J Nanomedicine Original Research BACKGROUND: Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are of great concern to the medical community and are difficult to cure. Therapies are available to treat such diseases, but all have drawbacks and are not specifically targeted to the site of disease. Chitosan is widely used in the biomedical community, including for orthopedic applications. The aim of the present study was to coat chitosan onto iron oxide nanoparticles and to determine its effect on the proliferation and differentiation of osteoblasts. METHODS: Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, and vibrating sample magnetometry. Uptake of nanoparticles by osteoblasts was studied by transmission electron microscopy and Prussian blue staining. Viability and proliferation of osteoblasts were measured in the presence of uncoated iron oxide magnetic nanoparticles or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, and extracellular calcium deposition was studied in the presence of the nanoparticles. RESULTS: Chitosan-coated iron oxide nanoparticles enhanced osteoblast proliferation, decreased cell membrane damage, and promoted cell differentiation, as indicated by an increase in alkaline phosphatase and extracellular calcium deposition. Chitosan-coated iron oxide nanoparticles showed good compatibility with osteoblasts. CONCLUSION: Further research is necessary to optimize magnetic nanoparticles for the treatment of bone disease. Dove Medical Press 2012 2012-10-25 /pmc/articles/PMC3484720/ /pubmed/23118539 http://dx.doi.org/10.2147/IJN.S34348 Text en © 2012 Shi et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Shi, Si-Feng
Jia, Jing-Fu
Guo, Xiao-Kui
Zhao, Ya-Ping
Chen, De-Sheng
Guo, Yong-Yuan
Cheng, Tao
Zhang, Xian-Long
Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_full Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_fullStr Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_full_unstemmed Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_short Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
title_sort biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484720/
https://www.ncbi.nlm.nih.gov/pubmed/23118539
http://dx.doi.org/10.2147/IJN.S34348
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