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In Vitro Site Selection of a Consensus Binding Site for the Drosophila melanogaster Tbx20 Homolog Midline

We employed in vitro site selection to identify a consensus binding sequence for the Drosophila melanogaster Tbx20 T-box transcription factor homolog Midline. We purified a bacterially expressed T-box DNA binding domain of Midline, and used it in four rounds of precipitation and polymerase-chain-rea...

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Detalles Bibliográficos
Autores principales:	Najand, Nima, Ryu, Jae-Ryeon, Brook, William J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485041/ https://www.ncbi.nlm.nih.gov/pubmed/23133562 http://dx.doi.org/10.1371/journal.pone.0048176

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