Cargando…

Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China

BACKGROUND: CD28 is one of a number of costimulatory molecules that play crucial roles in immune regulation and homeostasis. Accumulating evidence indicates that immune factors influence breast carcinogenesis. To clarify the relationships between polymorphisms in the CD28 gene and breast carcinogene...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Shuang, Zhang, Qing, Shen, Liming, Liu, Yanhong, Xu, Fengyan, Li, Dalin, Fu, Zhenkun, Yuan, Weiguang, Pang, Da, Li, Dianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485049/
https://www.ncbi.nlm.nih.gov/pubmed/23133541
http://dx.doi.org/10.1371/journal.pone.0048031
_version_ 1782248225487454208
author Chen, Shuang
Zhang, Qing
Shen, Liming
Liu, Yanhong
Xu, Fengyan
Li, Dalin
Fu, Zhenkun
Yuan, Weiguang
Pang, Da
Li, Dianjun
author_facet Chen, Shuang
Zhang, Qing
Shen, Liming
Liu, Yanhong
Xu, Fengyan
Li, Dalin
Fu, Zhenkun
Yuan, Weiguang
Pang, Da
Li, Dianjun
author_sort Chen, Shuang
collection PubMed
description BACKGROUND: CD28 is one of a number of costimulatory molecules that play crucial roles in immune regulation and homeostasis. Accumulating evidence indicates that immune factors influence breast carcinogenesis. To clarify the relationships between polymorphisms in the CD28 gene and breast carcinogenesis, a case-control study was conducted in women from Heilongjiang Province in northeast of China. METHODOLOGY/PRINCIPAL FINDINGS: Our research subjects consisted of 565 female patients with sporadic breast cancer and 605 age- and sex-matched healthy controls. In total, 12 single nucleotide polymorphisms (SNPs) in the CD28 gene were successfully determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The relationship between the CD28 variants and clinical features, including histological grade, tumor size, lymph node metastasis, human epidermal growth factor receptor 2 (C-erbB2), estrogen receptor (ER), progesterone receptor (PR), and tumor protein 53 (P53) status were analyzed. A statistically significant association was observed between rs3116496 and breast cancer risk under different genetic models (additive P = 0.0164, dominant P = 0.0042). Different distributions of the rs3116496 ‘T’ allele were found in patients and controls, which remained significant after correcting the P value for multiple testing using Haploview with 10,000 permutations (corrected P = 0.0384). In addition, significant associations were observed between rs3116487/rs3116494 (D’ = 1, r(2) = 0.99) and clinicopathological features such as C-erbB2 and ER status, in breast cancer patients. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that CD28 gene polymorphisms contribute to sporadic breast cancer risk and have a significant association with clinicopathological features in a northeast Chinese Han population.
format Online
Article
Text
id pubmed-3485049
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34850492012-11-06 Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China Chen, Shuang Zhang, Qing Shen, Liming Liu, Yanhong Xu, Fengyan Li, Dalin Fu, Zhenkun Yuan, Weiguang Pang, Da Li, Dianjun PLoS One Research Article BACKGROUND: CD28 is one of a number of costimulatory molecules that play crucial roles in immune regulation and homeostasis. Accumulating evidence indicates that immune factors influence breast carcinogenesis. To clarify the relationships between polymorphisms in the CD28 gene and breast carcinogenesis, a case-control study was conducted in women from Heilongjiang Province in northeast of China. METHODOLOGY/PRINCIPAL FINDINGS: Our research subjects consisted of 565 female patients with sporadic breast cancer and 605 age- and sex-matched healthy controls. In total, 12 single nucleotide polymorphisms (SNPs) in the CD28 gene were successfully determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The relationship between the CD28 variants and clinical features, including histological grade, tumor size, lymph node metastasis, human epidermal growth factor receptor 2 (C-erbB2), estrogen receptor (ER), progesterone receptor (PR), and tumor protein 53 (P53) status were analyzed. A statistically significant association was observed between rs3116496 and breast cancer risk under different genetic models (additive P = 0.0164, dominant P = 0.0042). Different distributions of the rs3116496 ‘T’ allele were found in patients and controls, which remained significant after correcting the P value for multiple testing using Haploview with 10,000 permutations (corrected P = 0.0384). In addition, significant associations were observed between rs3116487/rs3116494 (D’ = 1, r(2) = 0.99) and clinicopathological features such as C-erbB2 and ER status, in breast cancer patients. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that CD28 gene polymorphisms contribute to sporadic breast cancer risk and have a significant association with clinicopathological features in a northeast Chinese Han population. Public Library of Science 2012-10-25 /pmc/articles/PMC3485049/ /pubmed/23133541 http://dx.doi.org/10.1371/journal.pone.0048031 Text en © 2012 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Shuang
Zhang, Qing
Shen, Liming
Liu, Yanhong
Xu, Fengyan
Li, Dalin
Fu, Zhenkun
Yuan, Weiguang
Pang, Da
Li, Dianjun
Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China
title Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China
title_full Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China
title_fullStr Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China
title_full_unstemmed Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China
title_short Investigation of CD28 Gene Polymorphisms in Patients with Sporadic Breast Cancer in a Chinese Han Population in Northeast China
title_sort investigation of cd28 gene polymorphisms in patients with sporadic breast cancer in a chinese han population in northeast china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485049/
https://www.ncbi.nlm.nih.gov/pubmed/23133541
http://dx.doi.org/10.1371/journal.pone.0048031
work_keys_str_mv AT chenshuang investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT zhangqing investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT shenliming investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT liuyanhong investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT xufengyan investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT lidalin investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT fuzhenkun investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT yuanweiguang investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT pangda investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina
AT lidianjun investigationofcd28genepolymorphismsinpatientswithsporadicbreastcancerinachinesehanpopulationinnortheastchina