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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
An important route to understanding how proteins function at a mechanistic level is to have the structure of the target protein available, ideally at atomic resolution. Presently, there is only one way to capture such information as applied to integral membrane proteins (Figure 1), and the complexes...
Autores principales: | Li, Dianfan, Boland, Coilín, Aragao, David, Walsh, Kilian, Caffrey, Martin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485064/ https://www.ncbi.nlm.nih.gov/pubmed/22971942 http://dx.doi.org/10.3791/4001 |
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