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Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations

BACKGROUND: Neointimal formation in atherosclerosis has been subject for intense research. However, good animal models mimicking asymmetrical lesion formation in human subjects have been difficult to establish. The aim of this study was to develop a model which would lead to the formation of eccentr...

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Autores principales: Kivelä, Antti, Hartikainen, Juha, Ylä-Herttuala, Seppo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485168/
https://www.ncbi.nlm.nih.gov/pubmed/23075120
http://dx.doi.org/10.1186/1471-2261-12-91
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author Kivelä, Antti
Hartikainen, Juha
Ylä-Herttuala, Seppo
author_facet Kivelä, Antti
Hartikainen, Juha
Ylä-Herttuala, Seppo
author_sort Kivelä, Antti
collection PubMed
description BACKGROUND: Neointimal formation in atherosclerosis has been subject for intense research. However, good animal models mimicking asymmetrical lesion formation in human subjects have been difficult to establish. The aim of this study was to develop a model which would lead to the formation of eccentric lesions under macroscopically intact non-denuded endothelium. METHODS: We have developed a new collar model where we placed two cushions or dots inside the collar. Arterial lesions were characterized using histology and ultrasound methods. RESULTS: When this dotted collar was placed around carotid and femoral arteries it produced asymmetrical pressure on adventitia and a mild flow disturbance, and hence a change in shear stress. Our hypothesis was that this simple procedure would reproducibly produce asymmetrical lesions without any intraluminal manipulations. Intima/media ratio increased towards the distal end of the collar with the direction of blood flow under macroscopically intact endothelium. Macrophages preferentially accumulated in areas of the thickest neointima thus resembling early steps in human atherosclerotic plaque formation. Proliferating cells in these lesions and underlying media were scarce at eight weeks time point. CONCLUSION: The improved dotted collar model produces asymmetrical human-like atherosclerotic lesions in rabbits. This model should be useful in studies regarding the pathogenesis and formation of eccentric atherosclerotic lesions.
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spelling pubmed-34851682012-11-01 Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations Kivelä, Antti Hartikainen, Juha Ylä-Herttuala, Seppo BMC Cardiovasc Disord Research Article BACKGROUND: Neointimal formation in atherosclerosis has been subject for intense research. However, good animal models mimicking asymmetrical lesion formation in human subjects have been difficult to establish. The aim of this study was to develop a model which would lead to the formation of eccentric lesions under macroscopically intact non-denuded endothelium. METHODS: We have developed a new collar model where we placed two cushions or dots inside the collar. Arterial lesions were characterized using histology and ultrasound methods. RESULTS: When this dotted collar was placed around carotid and femoral arteries it produced asymmetrical pressure on adventitia and a mild flow disturbance, and hence a change in shear stress. Our hypothesis was that this simple procedure would reproducibly produce asymmetrical lesions without any intraluminal manipulations. Intima/media ratio increased towards the distal end of the collar with the direction of blood flow under macroscopically intact endothelium. Macrophages preferentially accumulated in areas of the thickest neointima thus resembling early steps in human atherosclerotic plaque formation. Proliferating cells in these lesions and underlying media were scarce at eight weeks time point. CONCLUSION: The improved dotted collar model produces asymmetrical human-like atherosclerotic lesions in rabbits. This model should be useful in studies regarding the pathogenesis and formation of eccentric atherosclerotic lesions. BioMed Central 2012-10-17 /pmc/articles/PMC3485168/ /pubmed/23075120 http://dx.doi.org/10.1186/1471-2261-12-91 Text en Copyright ©2012 Kivelä et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kivelä, Antti
Hartikainen, Juha
Ylä-Herttuala, Seppo
Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
title Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
title_full Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
title_fullStr Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
title_full_unstemmed Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
title_short Dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
title_sort dotted collar placed around carotid artery induces asymmetric neointimal lesion formation in rabbits without intravascular manipulations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485168/
https://www.ncbi.nlm.nih.gov/pubmed/23075120
http://dx.doi.org/10.1186/1471-2261-12-91
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