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Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae

New Zealand became geographically isolated about 80 million years ago and this separation gave rise to a unique native flora including four genera of legume, Carmichaelia, Clianthus and Montigena in the Carmichaelinae clade, tribe Galegeae, and Sophora, tribe Sophoreae, sub-family Papilionoideae. Te...

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Autores principales: Tan, Heng Wee, Weir, Bevan S., Carter, Noel, Heenan, Peter B., Ridgway, Hayley J., James, Euan K., Sprent, Janet I., Young, J. Peter W., Andrews, Mitchell
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485278/
https://www.ncbi.nlm.nih.gov/pubmed/23118889
http://dx.doi.org/10.1371/journal.pone.0047677
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author Tan, Heng Wee
Weir, Bevan S.
Carter, Noel
Heenan, Peter B.
Ridgway, Hayley J.
James, Euan K.
Sprent, Janet I.
Young, J. Peter W.
Andrews, Mitchell
author_facet Tan, Heng Wee
Weir, Bevan S.
Carter, Noel
Heenan, Peter B.
Ridgway, Hayley J.
James, Euan K.
Sprent, Janet I.
Young, J. Peter W.
Andrews, Mitchell
author_sort Tan, Heng Wee
collection PubMed
description New Zealand became geographically isolated about 80 million years ago and this separation gave rise to a unique native flora including four genera of legume, Carmichaelia, Clianthus and Montigena in the Carmichaelinae clade, tribe Galegeae, and Sophora, tribe Sophoreae, sub-family Papilionoideae. Ten bacterial strains isolated from NZ Carmichaelinae growing in natural ecosystems grouped close to the Mesorhizobium huakuii type strain in relation to their 16S rRNA and nifH gene sequences. However, the ten strains separated into four groups on the basis of their recA and glnII sequences: all groups were clearly distinct from all Mesorhizobium type strains. The ten strains separated into two groups on the basis of their nodA sequences but grouped closely together in relation to nodC sequences; all nodA and nodC sequences were novel. Seven strains selected and the M. huakuii type strain (isolated from Astragalus sinicus) produced functional nodules on Carmichaelia spp., Clianthus puniceus and A. sinicus but did not nodulate two Sophora species. We conclude that rhizobia closely related to M. huakuii on the basis of 16S rRNA and nifH gene sequences, but with variable recA and glnII genes and novel nodA and nodC genes, are common symbionts of NZ Carmichaelinae.
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spelling pubmed-34852782012-11-01 Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae Tan, Heng Wee Weir, Bevan S. Carter, Noel Heenan, Peter B. Ridgway, Hayley J. James, Euan K. Sprent, Janet I. Young, J. Peter W. Andrews, Mitchell PLoS One Research Article New Zealand became geographically isolated about 80 million years ago and this separation gave rise to a unique native flora including four genera of legume, Carmichaelia, Clianthus and Montigena in the Carmichaelinae clade, tribe Galegeae, and Sophora, tribe Sophoreae, sub-family Papilionoideae. Ten bacterial strains isolated from NZ Carmichaelinae growing in natural ecosystems grouped close to the Mesorhizobium huakuii type strain in relation to their 16S rRNA and nifH gene sequences. However, the ten strains separated into four groups on the basis of their recA and glnII sequences: all groups were clearly distinct from all Mesorhizobium type strains. The ten strains separated into two groups on the basis of their nodA sequences but grouped closely together in relation to nodC sequences; all nodA and nodC sequences were novel. Seven strains selected and the M. huakuii type strain (isolated from Astragalus sinicus) produced functional nodules on Carmichaelia spp., Clianthus puniceus and A. sinicus but did not nodulate two Sophora species. We conclude that rhizobia closely related to M. huakuii on the basis of 16S rRNA and nifH gene sequences, but with variable recA and glnII genes and novel nodA and nodC genes, are common symbionts of NZ Carmichaelinae. Public Library of Science 2012-10-31 /pmc/articles/PMC3485278/ /pubmed/23118889 http://dx.doi.org/10.1371/journal.pone.0047677 Text en © 2012 Tan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tan, Heng Wee
Weir, Bevan S.
Carter, Noel
Heenan, Peter B.
Ridgway, Hayley J.
James, Euan K.
Sprent, Janet I.
Young, J. Peter W.
Andrews, Mitchell
Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae
title Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae
title_full Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae
title_fullStr Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae
title_full_unstemmed Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae
title_short Rhizobia with 16S rRNA and nifH Similar to Mesorhizobium huakuii but Novel recA, glnII, nodA and nodC Genes Are Symbionts of New Zealand Carmichaelinae
title_sort rhizobia with 16s rrna and nifh similar to mesorhizobium huakuii but novel reca, glnii, noda and nodc genes are symbionts of new zealand carmichaelinae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485278/
https://www.ncbi.nlm.nih.gov/pubmed/23118889
http://dx.doi.org/10.1371/journal.pone.0047677
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