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A Modified Technique for Culturing Primary Fetal Rat Cortical Neurons

The study explored a modified primary culture system for fetal rat cortical neurons. Day E18 embryos from pregnant Sprague Dawley rats were microdissected under a stereoscope. To minimize enzymatic damage to the cultured neurons, we applied a sequential digestion protocol using papain and Dnase I. T...

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Detalles Bibliográficos
Autores principales:	Xu, Sui-Yi, Wu, Yong-Min, Ji, Zhong, Gao, Xiao-Ya, Pan, Su-Yue
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486623/ https://www.ncbi.nlm.nih.gov/pubmed/23193366 http://dx.doi.org/10.1155/2012/803930

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