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Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients

Natural killer (NK) cells are abundant in the liver and have been implicated in inducing hepatocellular damage in patients with chronic hepatitis B virus (HBV) infection. However, the role of NK cells in acute HBV infection remains to be elucidated. We comprehensively characterized NK cells and inve...

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Autores principales: Zhao, Juanjuan, Li, Yonggang, Jin, Lei, Zhang, Shuye, Fan, Rong, Sun, Yanling, Zhou, Chunbao, Shang, Qinghua, Li, Wengang, Zhang, Zheng, Wang, Fu-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486810/
https://www.ncbi.nlm.nih.gov/pubmed/23133672
http://dx.doi.org/10.1371/journal.pone.0049135
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author Zhao, Juanjuan
Li, Yonggang
Jin, Lei
Zhang, Shuye
Fan, Rong
Sun, Yanling
Zhou, Chunbao
Shang, Qinghua
Li, Wengang
Zhang, Zheng
Wang, Fu-Sheng
author_facet Zhao, Juanjuan
Li, Yonggang
Jin, Lei
Zhang, Shuye
Fan, Rong
Sun, Yanling
Zhou, Chunbao
Shang, Qinghua
Li, Wengang
Zhang, Zheng
Wang, Fu-Sheng
author_sort Zhao, Juanjuan
collection PubMed
description Natural killer (NK) cells are abundant in the liver and have been implicated in inducing hepatocellular damage in patients with chronic hepatitis B virus (HBV) infection. However, the role of NK cells in acute HBV infection remains to be elucidated. We comprehensively characterized NK cells and investigated their roles in HBV clearance and liver pathology in 19 chronic hepatitis B (CHB) patients and 21 acute hepatitis B (AHB) patients as well as 16 healthy subjects. It was found that NKp46(+) NK cells were enriched in the livers of AHB and CHB patients. We further found that peripheral NK cells from AHB patients expressed higher levels of activation receptors and lower levels of inhibitory receptors than those from CHB patients and HC subjects, thus displaying the increased cytolytic activity and interferon-γ production. NK cell activation levels were also correlated positively with serum alanine aminotransferase levels and negatively with plasma HBV DNA levels in AHB patients, which is further confirmed by the longitudinal follow-up of AHB patients. Serum pro-inflammatory cytokine and chemokine levels were also increased in AHB patients as compared with CHB and HC subjects. Thus, the concomitantly increased interferon-γ and cytotoxicity of NK cells were associated with liver injury and viral clearance in AHB patients.
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spelling pubmed-34868102012-11-06 Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients Zhao, Juanjuan Li, Yonggang Jin, Lei Zhang, Shuye Fan, Rong Sun, Yanling Zhou, Chunbao Shang, Qinghua Li, Wengang Zhang, Zheng Wang, Fu-Sheng PLoS One Research Article Natural killer (NK) cells are abundant in the liver and have been implicated in inducing hepatocellular damage in patients with chronic hepatitis B virus (HBV) infection. However, the role of NK cells in acute HBV infection remains to be elucidated. We comprehensively characterized NK cells and investigated their roles in HBV clearance and liver pathology in 19 chronic hepatitis B (CHB) patients and 21 acute hepatitis B (AHB) patients as well as 16 healthy subjects. It was found that NKp46(+) NK cells were enriched in the livers of AHB and CHB patients. We further found that peripheral NK cells from AHB patients expressed higher levels of activation receptors and lower levels of inhibitory receptors than those from CHB patients and HC subjects, thus displaying the increased cytolytic activity and interferon-γ production. NK cell activation levels were also correlated positively with serum alanine aminotransferase levels and negatively with plasma HBV DNA levels in AHB patients, which is further confirmed by the longitudinal follow-up of AHB patients. Serum pro-inflammatory cytokine and chemokine levels were also increased in AHB patients as compared with CHB and HC subjects. Thus, the concomitantly increased interferon-γ and cytotoxicity of NK cells were associated with liver injury and viral clearance in AHB patients. Public Library of Science 2012-11-01 /pmc/articles/PMC3486810/ /pubmed/23133672 http://dx.doi.org/10.1371/journal.pone.0049135 Text en © 2012 Zhao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhao, Juanjuan
Li, Yonggang
Jin, Lei
Zhang, Shuye
Fan, Rong
Sun, Yanling
Zhou, Chunbao
Shang, Qinghua
Li, Wengang
Zhang, Zheng
Wang, Fu-Sheng
Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients
title Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients
title_full Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients
title_fullStr Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients
title_full_unstemmed Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients
title_short Natural Killer Cells Are Characterized by the Concomitantly Increased Interferon-γ and Cytotoxicity in Acute Resolved Hepatitis B Patients
title_sort natural killer cells are characterized by the concomitantly increased interferon-γ and cytotoxicity in acute resolved hepatitis b patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486810/
https://www.ncbi.nlm.nih.gov/pubmed/23133672
http://dx.doi.org/10.1371/journal.pone.0049135
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