Next-Generation Sequencing of Human Mitochondrial Reference Genomes Uncovers High Heteroplasmy Frequency

We describe methods for rapid sequencing of the entire human mitochondrial genome (mtgenome), which involve long-range PCR for specific amplification of the mtgenome, pyrosequencing, quantitative mapping of sequence reads to identify sequence variants and heteroplasmy, as well as de novo sequence as...

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Detalles Bibliográficos
Autores principales: Sosa, Maria Ximena, Sivakumar, I. K. Ashok, Maragh, Samantha, Veeramachaneni, Vamsi, Hariharan, Ramesh, Parulekar, Minothi, Fredrikson, Karin M., Harkins, Timothy T., Lin, Jeffrey, Feldman, Andrew B., Tata, Pramila, Ehret, Georg B., Chakravarti, Aravinda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486893/
https://www.ncbi.nlm.nih.gov/pubmed/23133345
http://dx.doi.org/10.1371/journal.pcbi.1002737

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