Cargando…
Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil
Centricnemus leucogrammus is a weevil characteristic of European xerothermic habitats and steppes. The species was probably more widespread during the Pleistocene glaciations, while its current distribution is limited to “warm-stage refugia.” It may be regarded as a typical representative of flightl...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486996/ https://www.ncbi.nlm.nih.gov/pubmed/23065209 http://dx.doi.org/10.1007/s11033-012-2021-1 |
_version_ | 1782248430077214720 |
---|---|
author | Kajtoch, Łukasz Nadachowska-Brzyska, Krystyna Babik, Wiesław |
author_facet | Kajtoch, Łukasz Nadachowska-Brzyska, Krystyna Babik, Wiesław |
author_sort | Kajtoch, Łukasz |
collection | PubMed |
description | Centricnemus leucogrammus is a weevil characteristic of European xerothermic habitats and steppes. The species was probably more widespread during the Pleistocene glaciations, while its current distribution is limited to “warm-stage refugia.” It may be regarded as a typical representative of flightless xerothermophilous beetles. Previous studies concentrated on its genetic variation using mitochondrial genes. Here, we identified, tested and characterized 24 polymorphic microsatellite loci with the use of 454 sequencing of microsatellite enriched genomic libraries. The new set of loci will be used in studies on the population structure of this weevil and may provide valuable information for its conservation. |
format | Online Article Text |
id | pubmed-3486996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-34869962012-11-05 Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil Kajtoch, Łukasz Nadachowska-Brzyska, Krystyna Babik, Wiesław Mol Biol Rep Article Centricnemus leucogrammus is a weevil characteristic of European xerothermic habitats and steppes. The species was probably more widespread during the Pleistocene glaciations, while its current distribution is limited to “warm-stage refugia.” It may be regarded as a typical representative of flightless xerothermophilous beetles. Previous studies concentrated on its genetic variation using mitochondrial genes. Here, we identified, tested and characterized 24 polymorphic microsatellite loci with the use of 454 sequencing of microsatellite enriched genomic libraries. The new set of loci will be used in studies on the population structure of this weevil and may provide valuable information for its conservation. Springer Netherlands 2012-10-13 2012 /pmc/articles/PMC3486996/ /pubmed/23065209 http://dx.doi.org/10.1007/s11033-012-2021-1 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Article Kajtoch, Łukasz Nadachowska-Brzyska, Krystyna Babik, Wiesław Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil |
title | Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil |
title_full | Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil |
title_fullStr | Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil |
title_full_unstemmed | Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil |
title_short | Development and characterization of microsatellite loci in the Centricnemus leucogrammus weevil |
title_sort | development and characterization of microsatellite loci in the centricnemus leucogrammus weevil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486996/ https://www.ncbi.nlm.nih.gov/pubmed/23065209 http://dx.doi.org/10.1007/s11033-012-2021-1 |
work_keys_str_mv | AT kajtochłukasz developmentandcharacterizationofmicrosatellitelociinthecentricnemusleucogrammusweevil AT nadachowskabrzyskakrystyna developmentandcharacterizationofmicrosatellitelociinthecentricnemusleucogrammusweevil AT babikwiesław developmentandcharacterizationofmicrosatellitelociinthecentricnemusleucogrammusweevil |