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Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers

OBJECTIVES: Zornia diphylla (L.) Pers is an ethnomedical herb. The aim of the study is to scientifically verify the traditional use of Z. diphylla as an anticancer medicine. MATERIALS AND METHODS: Different extracts, fractions, and chemical isolates of the whole plant were screened for cytotoxicity...

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Autores principales: Arunkumar, R., Nair, S. Ajikumaran, Subramoniam, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487271/
https://www.ncbi.nlm.nih.gov/pubmed/23129958
http://dx.doi.org/10.4103/0976-500X.99420
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author Arunkumar, R.
Nair, S. Ajikumaran
Subramoniam, A.
author_facet Arunkumar, R.
Nair, S. Ajikumaran
Subramoniam, A.
author_sort Arunkumar, R.
collection PubMed
description OBJECTIVES: Zornia diphylla (L.) Pers is an ethnomedical herb. The aim of the study is to scientifically verify the traditional use of Z. diphylla as an anticancer medicine. MATERIALS AND METHODS: Different extracts, fractions, and chemical isolates of the whole plant were screened for cytotoxicity to Dalton's lymphoma ascites (DLA) cells by the Trypan blue exclusion method and MTT assay. Column chromatographic and preparative TLC techniques were used for the isolation of active fraction (AF) and active principle. Cytotoxicity of AF to different cell types was tested. The apoptotic activity of AF was evaluated by morphological observations, nuclear condensation, and comet assay. In vivo antitumor activity of AF was determined in DLA-challenged mice. Short-term (29 days) preliminary toxicity evaluation of AF was done in mice. RESULTS: n-Hexane extract (but not water and ethanol extracts) showed significant cytotoxicity. AF, isolated from n-hexane extract, induced apoptotic cell death (in vitro) to DLA cells, but not to normal thymocytes and macrophages. A steroid positive active principle was isolated which showed 100% cytotoxicity at 5 μg/mL level. Interestingly, AF (50 mg/kg) protected all the mice challenged with one million DLA cells/mouse. AF (up to 10 times higher than the therapeutic dose) did not exhibit any conspicuous adverse toxic symptoms in the toxicity evaluation. CONCLUSION: Z. diphylla (AF) showed promising in vitro and in vivo anticancer activity against DLA cells, and it was devoid of any toxicity to mice in short-term toxicity evaluation. The herb is promising for the development of a valuable anticancer medicine.
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spelling pubmed-34872712012-11-05 Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers Arunkumar, R. Nair, S. Ajikumaran Subramoniam, A. J Pharmacol Pharmacother Research Paper OBJECTIVES: Zornia diphylla (L.) Pers is an ethnomedical herb. The aim of the study is to scientifically verify the traditional use of Z. diphylla as an anticancer medicine. MATERIALS AND METHODS: Different extracts, fractions, and chemical isolates of the whole plant were screened for cytotoxicity to Dalton's lymphoma ascites (DLA) cells by the Trypan blue exclusion method and MTT assay. Column chromatographic and preparative TLC techniques were used for the isolation of active fraction (AF) and active principle. Cytotoxicity of AF to different cell types was tested. The apoptotic activity of AF was evaluated by morphological observations, nuclear condensation, and comet assay. In vivo antitumor activity of AF was determined in DLA-challenged mice. Short-term (29 days) preliminary toxicity evaluation of AF was done in mice. RESULTS: n-Hexane extract (but not water and ethanol extracts) showed significant cytotoxicity. AF, isolated from n-hexane extract, induced apoptotic cell death (in vitro) to DLA cells, but not to normal thymocytes and macrophages. A steroid positive active principle was isolated which showed 100% cytotoxicity at 5 μg/mL level. Interestingly, AF (50 mg/kg) protected all the mice challenged with one million DLA cells/mouse. AF (up to 10 times higher than the therapeutic dose) did not exhibit any conspicuous adverse toxic symptoms in the toxicity evaluation. CONCLUSION: Z. diphylla (AF) showed promising in vitro and in vivo anticancer activity against DLA cells, and it was devoid of any toxicity to mice in short-term toxicity evaluation. The herb is promising for the development of a valuable anticancer medicine. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3487271/ /pubmed/23129958 http://dx.doi.org/10.4103/0976-500X.99420 Text en Copyright: © Journal of Pharmacology and Pharmacotherapeutics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Arunkumar, R.
Nair, S. Ajikumaran
Subramoniam, A.
Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers
title Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers
title_full Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers
title_fullStr Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers
title_full_unstemmed Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers
title_short Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers
title_sort induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from zornia diphylla (l.) pers
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487271/
https://www.ncbi.nlm.nih.gov/pubmed/23129958
http://dx.doi.org/10.4103/0976-500X.99420
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