The Single T65S Mutation Generates Brighter Cyan Fluorescent Proteins with Increased Photostability and pH Insensitivity

Cyan fluorescent proteins (CFP) derived from Aequorea victoria GFP, carrying a tryptophan-based chromophore, are widely used as FRET donors in live cell fluorescence imaging experiments. Recently, several CFP variants with near-ultimate photophysical performances were obtained through a mix of site-...

Descripción completa

Detalles Bibliográficos
Autores principales: Fredj, Asma, Pasquier, Hélène, Demachy, Isabelle, Jonasson, Gabriella, Levy, Bernard, Derrien, Valérie, Bousmah, Yasmina, Manoussaris, Gallia, Wien, Frank, Ridard, Jacqueline, Erard, Marie, Merola, Fabienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487735/
https://www.ncbi.nlm.nih.gov/pubmed/23133673
http://dx.doi.org/10.1371/journal.pone.0049149

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487735/
https://www.ncbi.nlm.nih.gov/pubmed/23133673
http://dx.doi.org/10.1371/journal.pone.0049149

Ejemplares similares