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Extension of Yeast Chronological Lifespan by Methylamine

BACKGROUND: Chronological aging of yeast cells is commonly used as a model for aging of human post-mitotic cells. The yeast Saccharomyces cerevisiae grown on glucose in the presence of ammonium sulphate is mainly used in yeast aging research. We have analyzed chronological aging of the yeast Hansenu...

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Autores principales: Kumar, Sanjeev, Lefevre, Sophie D., Veenhuis, Marten, van der Klei, Ida J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487785/
https://www.ncbi.nlm.nih.gov/pubmed/23133668
http://dx.doi.org/10.1371/journal.pone.0048982
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author Kumar, Sanjeev
Lefevre, Sophie D.
Veenhuis, Marten
van der Klei, Ida J.
author_facet Kumar, Sanjeev
Lefevre, Sophie D.
Veenhuis, Marten
van der Klei, Ida J.
author_sort Kumar, Sanjeev
collection PubMed
description BACKGROUND: Chronological aging of yeast cells is commonly used as a model for aging of human post-mitotic cells. The yeast Saccharomyces cerevisiae grown on glucose in the presence of ammonium sulphate is mainly used in yeast aging research. We have analyzed chronological aging of the yeast Hansenula polymorpha grown at conditions that require primary peroxisome metabolism for growth. METHODOLOGY/PRINCIPAL FINDINGS: The chronological lifespan of H. polymorpha is strongly enhanced when cells are grown on methanol or ethanol, metabolized by peroxisome enzymes, relative to growth on glucose that does not require peroxisomes. The short lifespan of H. polymorpha on glucose is mainly due to medium acidification, whereas most likely ROS do not play an important role. Growth of cells on methanol/methylamine instead of methanol/ammonium sulphate resulted in further lifespan enhancement. This was unrelated to medium acidification. We show that oxidation of methylamine by peroxisomal amine oxidase at carbon starvation conditions is responsible for lifespan extension. The methylamine oxidation product formaldehyde is further oxidized resulting in NADH generation, which contributes to increased ATP generation and reduction of ROS levels in the stationary phase. CONCLUSION/SIGNIFICANCE: We conclude that primary peroxisome metabolism enhanced chronological lifespan of H. polymorpha. Moreover, the possibility to generate NADH at carbon starvation conditions by an organic nitrogen source supports further extension of the lifespan of the cell. Consequently, the interpretation of CLS analyses in yeast should include possible effects on the energy status of the cell.
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spelling pubmed-34877852012-11-06 Extension of Yeast Chronological Lifespan by Methylamine Kumar, Sanjeev Lefevre, Sophie D. Veenhuis, Marten van der Klei, Ida J. PLoS One Research Article BACKGROUND: Chronological aging of yeast cells is commonly used as a model for aging of human post-mitotic cells. The yeast Saccharomyces cerevisiae grown on glucose in the presence of ammonium sulphate is mainly used in yeast aging research. We have analyzed chronological aging of the yeast Hansenula polymorpha grown at conditions that require primary peroxisome metabolism for growth. METHODOLOGY/PRINCIPAL FINDINGS: The chronological lifespan of H. polymorpha is strongly enhanced when cells are grown on methanol or ethanol, metabolized by peroxisome enzymes, relative to growth on glucose that does not require peroxisomes. The short lifespan of H. polymorpha on glucose is mainly due to medium acidification, whereas most likely ROS do not play an important role. Growth of cells on methanol/methylamine instead of methanol/ammonium sulphate resulted in further lifespan enhancement. This was unrelated to medium acidification. We show that oxidation of methylamine by peroxisomal amine oxidase at carbon starvation conditions is responsible for lifespan extension. The methylamine oxidation product formaldehyde is further oxidized resulting in NADH generation, which contributes to increased ATP generation and reduction of ROS levels in the stationary phase. CONCLUSION/SIGNIFICANCE: We conclude that primary peroxisome metabolism enhanced chronological lifespan of H. polymorpha. Moreover, the possibility to generate NADH at carbon starvation conditions by an organic nitrogen source supports further extension of the lifespan of the cell. Consequently, the interpretation of CLS analyses in yeast should include possible effects on the energy status of the cell. Public Library of Science 2012-11-02 /pmc/articles/PMC3487785/ /pubmed/23133668 http://dx.doi.org/10.1371/journal.pone.0048982 Text en © 2012 Kumar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kumar, Sanjeev
Lefevre, Sophie D.
Veenhuis, Marten
van der Klei, Ida J.
Extension of Yeast Chronological Lifespan by Methylamine
title Extension of Yeast Chronological Lifespan by Methylamine
title_full Extension of Yeast Chronological Lifespan by Methylamine
title_fullStr Extension of Yeast Chronological Lifespan by Methylamine
title_full_unstemmed Extension of Yeast Chronological Lifespan by Methylamine
title_short Extension of Yeast Chronological Lifespan by Methylamine
title_sort extension of yeast chronological lifespan by methylamine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487785/
https://www.ncbi.nlm.nih.gov/pubmed/23133668
http://dx.doi.org/10.1371/journal.pone.0048982
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