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Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses
BACKGROUND: Little is known about the potential of Brachypodium distachyon as a model for low temperature stress responses in Pooideae. The ice recrystallization inhibition protein (IRIP) genes, fructosyltransferase (FST) genes, and many C-repeat binding factor (CBF) genes are Pooideae specific and...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487962/ https://www.ncbi.nlm.nih.gov/pubmed/22569006 http://dx.doi.org/10.1186/1471-2229-12-65 |
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author | Li, Chuan Rudi, Heidi Stockinger, Eric J Cheng, Hongmei Cao, Moju Fox, Samuel E Mockler, Todd C Westereng, Bjørge Fjellheim, Siri Rognli, Odd Arne Sandve, Simen R |
author_facet | Li, Chuan Rudi, Heidi Stockinger, Eric J Cheng, Hongmei Cao, Moju Fox, Samuel E Mockler, Todd C Westereng, Bjørge Fjellheim, Siri Rognli, Odd Arne Sandve, Simen R |
author_sort | Li, Chuan |
collection | PubMed |
description | BACKGROUND: Little is known about the potential of Brachypodium distachyon as a model for low temperature stress responses in Pooideae. The ice recrystallization inhibition protein (IRIP) genes, fructosyltransferase (FST) genes, and many C-repeat binding factor (CBF) genes are Pooideae specific and important in low temperature responses. Here we used comparative analyses to study conservation and evolution of these gene families in B. distachyon to better understand its potential as a model species for agriculturally important temperate grasses. RESULTS: Brachypodium distachyon contains cold responsive IRIP genes which have evolved through Brachypodium specific gene family expansions. A large cold responsive CBF3 subfamily was identified in B. distachyon, while CBF4 homologs are absent from the genome. No B. distachyon FST gene homologs encode typical core Pooideae FST-motifs and low temperature induced fructan accumulation was dramatically different in B. distachyon compared to core Pooideae species. CONCLUSIONS: We conclude that B. distachyon can serve as an interesting model for specific molecular mechanisms involved in low temperature responses in core Pooideae species. However, the evolutionary history of key genes involved in low temperature responses has been different in Brachypodium and core Pooideae species. These differences limit the use of B. distachyon as a model for holistic studies relevant for agricultural core Pooideae species. |
format | Online Article Text |
id | pubmed-3487962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34879622012-11-03 Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses Li, Chuan Rudi, Heidi Stockinger, Eric J Cheng, Hongmei Cao, Moju Fox, Samuel E Mockler, Todd C Westereng, Bjørge Fjellheim, Siri Rognli, Odd Arne Sandve, Simen R BMC Plant Biol Research Article BACKGROUND: Little is known about the potential of Brachypodium distachyon as a model for low temperature stress responses in Pooideae. The ice recrystallization inhibition protein (IRIP) genes, fructosyltransferase (FST) genes, and many C-repeat binding factor (CBF) genes are Pooideae specific and important in low temperature responses. Here we used comparative analyses to study conservation and evolution of these gene families in B. distachyon to better understand its potential as a model species for agriculturally important temperate grasses. RESULTS: Brachypodium distachyon contains cold responsive IRIP genes which have evolved through Brachypodium specific gene family expansions. A large cold responsive CBF3 subfamily was identified in B. distachyon, while CBF4 homologs are absent from the genome. No B. distachyon FST gene homologs encode typical core Pooideae FST-motifs and low temperature induced fructan accumulation was dramatically different in B. distachyon compared to core Pooideae species. CONCLUSIONS: We conclude that B. distachyon can serve as an interesting model for specific molecular mechanisms involved in low temperature responses in core Pooideae species. However, the evolutionary history of key genes involved in low temperature responses has been different in Brachypodium and core Pooideae species. These differences limit the use of B. distachyon as a model for holistic studies relevant for agricultural core Pooideae species. BioMed Central 2012-05-08 /pmc/articles/PMC3487962/ /pubmed/22569006 http://dx.doi.org/10.1186/1471-2229-12-65 Text en Copyright ©2012 Li et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Chuan Rudi, Heidi Stockinger, Eric J Cheng, Hongmei Cao, Moju Fox, Samuel E Mockler, Todd C Westereng, Bjørge Fjellheim, Siri Rognli, Odd Arne Sandve, Simen R Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses |
title | Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses |
title_full | Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses |
title_fullStr | Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses |
title_full_unstemmed | Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses |
title_short | Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses |
title_sort | comparative analyses reveal potential uses of brachypodium distachyon as a model for cold stress responses in temperate grasses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487962/ https://www.ncbi.nlm.nih.gov/pubmed/22569006 http://dx.doi.org/10.1186/1471-2229-12-65 |
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