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Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes

SUMOylation of transcription factors and chromatin proteins is in many cases a negative mark that recruits factors that repress gene expression. In this study, we determined the occupancy of Small Ubiquitin-like MOdifier (SUMO)-1 on chromatin in HeLa cells by use of chromatin affinity purification c...

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Autores principales: Liu, Hui-wen, Zhang, Jie, Heine, George F., Arora, Mansi, Gulcin Ozer, Hatice, Onti-Srinivasan, Raghuram, Huang, Kun, Parvin, Jeffrey D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488252/
https://www.ncbi.nlm.nih.gov/pubmed/22941651
http://dx.doi.org/10.1093/nar/gks819
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author Liu, Hui-wen
Zhang, Jie
Heine, George F.
Arora, Mansi
Gulcin Ozer, Hatice
Onti-Srinivasan, Raghuram
Huang, Kun
Parvin, Jeffrey D.
author_facet Liu, Hui-wen
Zhang, Jie
Heine, George F.
Arora, Mansi
Gulcin Ozer, Hatice
Onti-Srinivasan, Raghuram
Huang, Kun
Parvin, Jeffrey D.
author_sort Liu, Hui-wen
collection PubMed
description SUMOylation of transcription factors and chromatin proteins is in many cases a negative mark that recruits factors that repress gene expression. In this study, we determined the occupancy of Small Ubiquitin-like MOdifier (SUMO)-1 on chromatin in HeLa cells by use of chromatin affinity purification coupled with next-generation sequencing. We found SUMO-1 localization on chromatin was dynamic throughout the cell cycle. Surprisingly, we observed that from G1 through late S phase, but not during mitosis, SUMO-1 marks the chromatin just upstream of the transcription start site on many of the most active housekeeping genes, including genes encoding translation factors and ribosomal subunit proteins. Moreover, we found that SUMO-1 distribution on promoters was correlated with H3K4me3, another general chromatin activation mark. Depletion of SUMO-1 resulted in downregulation of the genes that were marked by SUMO-1 at their promoters during interphase, supporting the concept that the marking of promoters by SUMO-1 is associated with transcriptional activation of genes involved in ribosome biosynthesis and in the protein translation process.
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spelling pubmed-34882522012-11-06 Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes Liu, Hui-wen Zhang, Jie Heine, George F. Arora, Mansi Gulcin Ozer, Hatice Onti-Srinivasan, Raghuram Huang, Kun Parvin, Jeffrey D. Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics SUMOylation of transcription factors and chromatin proteins is in many cases a negative mark that recruits factors that repress gene expression. In this study, we determined the occupancy of Small Ubiquitin-like MOdifier (SUMO)-1 on chromatin in HeLa cells by use of chromatin affinity purification coupled with next-generation sequencing. We found SUMO-1 localization on chromatin was dynamic throughout the cell cycle. Surprisingly, we observed that from G1 through late S phase, but not during mitosis, SUMO-1 marks the chromatin just upstream of the transcription start site on many of the most active housekeeping genes, including genes encoding translation factors and ribosomal subunit proteins. Moreover, we found that SUMO-1 distribution on promoters was correlated with H3K4me3, another general chromatin activation mark. Depletion of SUMO-1 resulted in downregulation of the genes that were marked by SUMO-1 at their promoters during interphase, supporting the concept that the marking of promoters by SUMO-1 is associated with transcriptional activation of genes involved in ribosome biosynthesis and in the protein translation process. Oxford University Press 2012-11 2012-08-30 /pmc/articles/PMC3488252/ /pubmed/22941651 http://dx.doi.org/10.1093/nar/gks819 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Liu, Hui-wen
Zhang, Jie
Heine, George F.
Arora, Mansi
Gulcin Ozer, Hatice
Onti-Srinivasan, Raghuram
Huang, Kun
Parvin, Jeffrey D.
Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes
title Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes
title_full Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes
title_fullStr Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes
title_full_unstemmed Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes
title_short Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes
title_sort chromatin modification by sumo-1 stimulates the promoters of translation machinery genes
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488252/
https://www.ncbi.nlm.nih.gov/pubmed/22941651
http://dx.doi.org/10.1093/nar/gks819
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