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Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats

BACKGROUND: Nuna Kadugu (NK), a Siddha medicine prepared from leaves and fruits of Morinda Pubescens, used for the treatment of various skin diseases. Though NK has been widely used for several decades, no scientific report was available on its safety. Present study was undertaken to demonstrate the...

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Autores principales: Ramaswamy, Ramaswamy Selvaratnam, Prathyusha, Nettam, Saranya, Ruthiramoorthi, Sumathy, Haridass, Mohanavalli, Kutuva Tulasi, Priya, Raju Jyothi, Venkhatesh, Jayakothanda Ramaswamy, Babu, Chidambaram Saravana, Manickavasakam, Kumarasamy, Thanikachalam, Sadagopan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488310/
https://www.ncbi.nlm.nih.gov/pubmed/23088610
http://dx.doi.org/10.1186/1472-6882-12-190
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author Ramaswamy, Ramaswamy Selvaratnam
Prathyusha, Nettam
Saranya, Ruthiramoorthi
Sumathy, Haridass
Mohanavalli, Kutuva Tulasi
Priya, Raju Jyothi
Venkhatesh, Jayakothanda Ramaswamy
Babu, Chidambaram Saravana
Manickavasakam, Kumarasamy
Thanikachalam, Sadagopan
author_facet Ramaswamy, Ramaswamy Selvaratnam
Prathyusha, Nettam
Saranya, Ruthiramoorthi
Sumathy, Haridass
Mohanavalli, Kutuva Tulasi
Priya, Raju Jyothi
Venkhatesh, Jayakothanda Ramaswamy
Babu, Chidambaram Saravana
Manickavasakam, Kumarasamy
Thanikachalam, Sadagopan
author_sort Ramaswamy, Ramaswamy Selvaratnam
collection PubMed
description BACKGROUND: Nuna Kadugu (NK), a Siddha medicine prepared from leaves and fruits of Morinda Pubescens, used for the treatment of various skin diseases. Though NK has been widely used for several decades, no scientific report was available on its safety. Present study was undertaken to demonstrate the oral toxicity of NK in Sprague Dawley rats. METHODS: Acute and 28-day repeated oral toxicity studies were performed following OECD test guidelines 423 and 407, respectively, with minor modifications. In acute oral toxicity study, NK was administered at 2000mg/kg b.wt., p.o and animals were observed for toxic signs at 0, 0.5, 1, 4, 24 h and for next 14 days. Gross pathology was performed at the end of the study. In repeated dose, the 28- day oral toxicity study, NK was administered at 300, 600 and 900 mg/kg b.wt./p.o/day. Two satellite groups (control and high dose) were also maintained to determine the delayed onset toxicity of NK. Animals were observed for mortality, morbidity, body weight changes, feed and water intake. Haematology, clinical biochemistry, electrolytes, gross pathology, relative organ weight and histopathological examination were performed. RESULTS: In acute toxicity study, no treatment related death or toxic signs were observed with NK administration. In the repeated dose study, no significant differences in body weight changes, food / water intake, haematology, clinical biochemistry and electrolytes content were observed between control and NK groups. No gross pathological findings and difference in relative organ weights were observed between control and NK treated rats. Histopathological examination revealed no abnormalities with NK treatment. CONCLUSION: Acute study reveals that the LD(50) of NK is greater than 2000mg/kg, b.wt. in fasted female rats and can be classified as Category 5. 28-day repeated oral toxicity demonstrates that the No Observed Adverse Effect Level of NK is greater than 900 mg/kg b.wt./day, p.o in rats. There were no delayed effects in NK satellite group. In conclusion, NK was found to be non-toxic in the tested doses and experimental conditions.
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spelling pubmed-34883102012-11-04 Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats Ramaswamy, Ramaswamy Selvaratnam Prathyusha, Nettam Saranya, Ruthiramoorthi Sumathy, Haridass Mohanavalli, Kutuva Tulasi Priya, Raju Jyothi Venkhatesh, Jayakothanda Ramaswamy Babu, Chidambaram Saravana Manickavasakam, Kumarasamy Thanikachalam, Sadagopan BMC Complement Altern Med Research Article BACKGROUND: Nuna Kadugu (NK), a Siddha medicine prepared from leaves and fruits of Morinda Pubescens, used for the treatment of various skin diseases. Though NK has been widely used for several decades, no scientific report was available on its safety. Present study was undertaken to demonstrate the oral toxicity of NK in Sprague Dawley rats. METHODS: Acute and 28-day repeated oral toxicity studies were performed following OECD test guidelines 423 and 407, respectively, with minor modifications. In acute oral toxicity study, NK was administered at 2000mg/kg b.wt., p.o and animals were observed for toxic signs at 0, 0.5, 1, 4, 24 h and for next 14 days. Gross pathology was performed at the end of the study. In repeated dose, the 28- day oral toxicity study, NK was administered at 300, 600 and 900 mg/kg b.wt./p.o/day. Two satellite groups (control and high dose) were also maintained to determine the delayed onset toxicity of NK. Animals were observed for mortality, morbidity, body weight changes, feed and water intake. Haematology, clinical biochemistry, electrolytes, gross pathology, relative organ weight and histopathological examination were performed. RESULTS: In acute toxicity study, no treatment related death or toxic signs were observed with NK administration. In the repeated dose study, no significant differences in body weight changes, food / water intake, haematology, clinical biochemistry and electrolytes content were observed between control and NK groups. No gross pathological findings and difference in relative organ weights were observed between control and NK treated rats. Histopathological examination revealed no abnormalities with NK treatment. CONCLUSION: Acute study reveals that the LD(50) of NK is greater than 2000mg/kg, b.wt. in fasted female rats and can be classified as Category 5. 28-day repeated oral toxicity demonstrates that the No Observed Adverse Effect Level of NK is greater than 900 mg/kg b.wt./day, p.o in rats. There were no delayed effects in NK satellite group. In conclusion, NK was found to be non-toxic in the tested doses and experimental conditions. BioMed Central 2012-10-22 /pmc/articles/PMC3488310/ /pubmed/23088610 http://dx.doi.org/10.1186/1472-6882-12-190 Text en Copyright ©2012 Ramaswamy et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ramaswamy, Ramaswamy Selvaratnam
Prathyusha, Nettam
Saranya, Ruthiramoorthi
Sumathy, Haridass
Mohanavalli, Kutuva Tulasi
Priya, Raju Jyothi
Venkhatesh, Jayakothanda Ramaswamy
Babu, Chidambaram Saravana
Manickavasakam, Kumarasamy
Thanikachalam, Sadagopan
Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
title Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
title_full Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
title_fullStr Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
title_full_unstemmed Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
title_short Acute toxicity and the 28-day repeated dose study of a Siddha medicine Nuna Kadugu in rats
title_sort acute toxicity and the 28-day repeated dose study of a siddha medicine nuna kadugu in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488310/
https://www.ncbi.nlm.nih.gov/pubmed/23088610
http://dx.doi.org/10.1186/1472-6882-12-190
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