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Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model

OBJECTIVE: To evaluate the effect of calcium supplementation on spinal bone fusion in ovariectomized (OVX) rats. METHODS: Sixteen female Sprague Dawley rats underwent bilateral ovariectomy at 12 weeks of age to induce osteoporosis and were randomly assigned to two groups : control group (n=8) and ca...

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Autores principales: Cho, Jae-Hoon, Cho, Dae-Chul, Yu, Song-Hee, Jeon, Young-Hoon, Sung, Joo-Kyung, Kim, Kyoung-Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Neurosurgical Society 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488633/
https://www.ncbi.nlm.nih.gov/pubmed/23133713
http://dx.doi.org/10.3340/jkns.2012.52.4.281
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author Cho, Jae-Hoon
Cho, Dae-Chul
Yu, Song-Hee
Jeon, Young-Hoon
Sung, Joo-Kyung
Kim, Kyoung-Tae
author_facet Cho, Jae-Hoon
Cho, Dae-Chul
Yu, Song-Hee
Jeon, Young-Hoon
Sung, Joo-Kyung
Kim, Kyoung-Tae
author_sort Cho, Jae-Hoon
collection PubMed
description OBJECTIVE: To evaluate the effect of calcium supplementation on spinal bone fusion in ovariectomized (OVX) rats. METHODS: Sixteen female Sprague Dawley rats underwent bilateral ovariectomy at 12 weeks of age to induce osteoporosis and were randomly assigned to two groups : control group (n=8) and calcium-supplemented group (OVX-Ca, n=8). Autologous spinal bone fusion surgery was performed on both groups 8 weeks later. After fusion surgery, the OVX-Ca group was supplemented with calcium in drinking water for 8 weeks. Blood was obtained 4 and 8 weeks after fusion surgery. Eight weeks after fusion surgery, the rats were euthanized and the L4-5 spine removed. Bone fusion status and fusion volume were evaluated by manual palpation and three-dimensional computed tomography. RESULTS: The mean fusion volume in the L4-5 spine was significantly greater in the OVX-Ca group (71.80±8.06 mm(3)) than in controls (35.34±8.24 mm(3)) (p<0.01). The level of osteocalcin, a bone formation marker, was higher in OVX-Ca rats than in controls 4 weeks (610.08±10.41 vs. 551.61±12.34 ng/mL) and 8 weeks (552.05±19.67 vs. 502.98±22.76 ng/mL) after fusion surgery (p<0.05). The level of C-terminal telopeptide fragment of type I collagen, a bone resorption marker, was significantly lower in OVX-Ca rats than in controls 4 weeks (77.07±12.57 vs. 101.75±7.20 ng/mL) and 8 weeks (69.58±2.45 vs. 77.15±4.10 ng/mL) after fusion surgery (p<0.05). A mechanical strength test showed that the L4-5 vertebrae in the OVX-Ca group withstood a 50% higher maximal load compared with the controls (p<0.01). CONCLUSION: Dietary calcium given to OVX rats after lumbar fusion surgery improved fusion volume and mechanical strength in an ovariectomized rat model.
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spelling pubmed-34886332012-11-06 Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model Cho, Jae-Hoon Cho, Dae-Chul Yu, Song-Hee Jeon, Young-Hoon Sung, Joo-Kyung Kim, Kyoung-Tae J Korean Neurosurg Soc Laboratory Investigation OBJECTIVE: To evaluate the effect of calcium supplementation on spinal bone fusion in ovariectomized (OVX) rats. METHODS: Sixteen female Sprague Dawley rats underwent bilateral ovariectomy at 12 weeks of age to induce osteoporosis and were randomly assigned to two groups : control group (n=8) and calcium-supplemented group (OVX-Ca, n=8). Autologous spinal bone fusion surgery was performed on both groups 8 weeks later. After fusion surgery, the OVX-Ca group was supplemented with calcium in drinking water for 8 weeks. Blood was obtained 4 and 8 weeks after fusion surgery. Eight weeks after fusion surgery, the rats were euthanized and the L4-5 spine removed. Bone fusion status and fusion volume were evaluated by manual palpation and three-dimensional computed tomography. RESULTS: The mean fusion volume in the L4-5 spine was significantly greater in the OVX-Ca group (71.80±8.06 mm(3)) than in controls (35.34±8.24 mm(3)) (p<0.01). The level of osteocalcin, a bone formation marker, was higher in OVX-Ca rats than in controls 4 weeks (610.08±10.41 vs. 551.61±12.34 ng/mL) and 8 weeks (552.05±19.67 vs. 502.98±22.76 ng/mL) after fusion surgery (p<0.05). The level of C-terminal telopeptide fragment of type I collagen, a bone resorption marker, was significantly lower in OVX-Ca rats than in controls 4 weeks (77.07±12.57 vs. 101.75±7.20 ng/mL) and 8 weeks (69.58±2.45 vs. 77.15±4.10 ng/mL) after fusion surgery (p<0.05). A mechanical strength test showed that the L4-5 vertebrae in the OVX-Ca group withstood a 50% higher maximal load compared with the controls (p<0.01). CONCLUSION: Dietary calcium given to OVX rats after lumbar fusion surgery improved fusion volume and mechanical strength in an ovariectomized rat model. The Korean Neurosurgical Society 2012-10 2012-10-22 /pmc/articles/PMC3488633/ /pubmed/23133713 http://dx.doi.org/10.3340/jkns.2012.52.4.281 Text en Copyright © 2012 The Korean Neurosurgical Society http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Laboratory Investigation
Cho, Jae-Hoon
Cho, Dae-Chul
Yu, Song-Hee
Jeon, Young-Hoon
Sung, Joo-Kyung
Kim, Kyoung-Tae
Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
title Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
title_full Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
title_fullStr Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
title_full_unstemmed Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
title_short Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
title_sort effect of dietary calcium on spinal bone fusion in an ovariectomized rat model
topic Laboratory Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488633/
https://www.ncbi.nlm.nih.gov/pubmed/23133713
http://dx.doi.org/10.3340/jkns.2012.52.4.281
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