Experimental phasing using zinc anomalous scattering

Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged o...

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Detalles Bibliográficos
Autores principales: Cha, Sun-Shin, An, Young Jun, Jeong, Chang-Sook, Kim, Min-Kyu, Lee, Sung-Gyu, Lee, Kwang-Hoon, Oh, Byung-Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2012
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489106/
https://www.ncbi.nlm.nih.gov/pubmed/22948927
http://dx.doi.org/10.1107/S0907444912024420
Descripción
Sumario:Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

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