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Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study

BACKGROUND: Cigarette smoke is associated with decreased female fertility, causing damage to ovarian function and disturbing follicle development. However, the effects of cigarette toxicants on ovarian function depend on duration and intensity of exposure. The aim of this study was to assess the eff...

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Autores principales: Paixão, Larissa LO, Gaspar-Reis, Rejane P, Gonzalez, Gabrielle PL, Santos, Aline S, Santana, Aluana C, Santos, Rachel MM, Spritzer, Poli Mara, Nascimento-Saba, Celly Cristina A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489515/
https://www.ncbi.nlm.nih.gov/pubmed/22995067
http://dx.doi.org/10.1186/1757-2215-5-25
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author Paixão, Larissa LO
Gaspar-Reis, Rejane P
Gonzalez, Gabrielle PL
Santos, Aline S
Santana, Aluana C
Santos, Rachel MM
Spritzer, Poli Mara
Nascimento-Saba, Celly Cristina A
author_facet Paixão, Larissa LO
Gaspar-Reis, Rejane P
Gonzalez, Gabrielle PL
Santos, Aline S
Santana, Aluana C
Santos, Rachel MM
Spritzer, Poli Mara
Nascimento-Saba, Celly Cristina A
author_sort Paixão, Larissa LO
collection PubMed
description BACKGROUND: Cigarette smoke is associated with decreased female fertility, causing damage to ovarian function and disturbing follicle development. However, the effects of cigarette toxicants on ovarian function depend on duration and intensity of exposure. The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. METHODS: Ten female Swiss mice aged 35 days were exposed to tobacco smoke from 3R4F reference research cigarettes. They were exposed to an automatic smoking machine 8 h/day, 7 days/week for 15 days. Ten age-matched controls were kept in a different room and exposed to ambient air. At the end of 15 days, five mice in each group were euthanized and the ovaries were analyzed for follicular morphometry and granulosa cell count. The remaining animals were kept for an additional 30 days for further analysis as an ex-smoker group and control group. Comparison between the two groups was evaluated by the Student’s t-test or a two-way ANOVA followed by Bonferroni post-test was applied for multiple comparisons. RESULTS: We found that cigarette smoke impaired antral follicular growth even after exposure cessation (p < 0.001). Both smoking and ex-smoking groups exhibited similar follicle diameter. However, at the same follicular stage, the number of granulosa cells was smaller in the ex-smoking group compared to smoking animals (p < 0.001). This was associated with increased oocyte diameter in ex-smoking animals compared to smoking animals (p < 0.01). CONCLUSIONS: The negative effects of cigarette smoking seem to last even after exposure has been interrupted. Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates.
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spelling pubmed-34895152012-11-06 Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study Paixão, Larissa LO Gaspar-Reis, Rejane P Gonzalez, Gabrielle PL Santos, Aline S Santana, Aluana C Santos, Rachel MM Spritzer, Poli Mara Nascimento-Saba, Celly Cristina A J Ovarian Res Research BACKGROUND: Cigarette smoke is associated with decreased female fertility, causing damage to ovarian function and disturbing follicle development. However, the effects of cigarette toxicants on ovarian function depend on duration and intensity of exposure. The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. METHODS: Ten female Swiss mice aged 35 days were exposed to tobacco smoke from 3R4F reference research cigarettes. They were exposed to an automatic smoking machine 8 h/day, 7 days/week for 15 days. Ten age-matched controls were kept in a different room and exposed to ambient air. At the end of 15 days, five mice in each group were euthanized and the ovaries were analyzed for follicular morphometry and granulosa cell count. The remaining animals were kept for an additional 30 days for further analysis as an ex-smoker group and control group. Comparison between the two groups was evaluated by the Student’s t-test or a two-way ANOVA followed by Bonferroni post-test was applied for multiple comparisons. RESULTS: We found that cigarette smoke impaired antral follicular growth even after exposure cessation (p < 0.001). Both smoking and ex-smoking groups exhibited similar follicle diameter. However, at the same follicular stage, the number of granulosa cells was smaller in the ex-smoking group compared to smoking animals (p < 0.001). This was associated with increased oocyte diameter in ex-smoking animals compared to smoking animals (p < 0.01). CONCLUSIONS: The negative effects of cigarette smoking seem to last even after exposure has been interrupted. Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates. BioMed Central 2012-09-20 /pmc/articles/PMC3489515/ /pubmed/22995067 http://dx.doi.org/10.1186/1757-2215-5-25 Text en Copyright ©2012 Paixão et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Paixão, Larissa LO
Gaspar-Reis, Rejane P
Gonzalez, Gabrielle PL
Santos, Aline S
Santana, Aluana C
Santos, Rachel MM
Spritzer, Poli Mara
Nascimento-Saba, Celly Cristina A
Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study
title Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study
title_full Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study
title_fullStr Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study
title_full_unstemmed Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study
title_short Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study
title_sort cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young swiss mice: an experimental study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489515/
https://www.ncbi.nlm.nih.gov/pubmed/22995067
http://dx.doi.org/10.1186/1757-2215-5-25
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