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Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay

BACKGROUND: Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoa...

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Autores principales: Czerwinski, Sarah L, Lyon, Andrew W, Skorobohach, Brian, Léguillette, Renaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489562/
https://www.ncbi.nlm.nih.gov/pubmed/22909398
http://dx.doi.org/10.1186/1746-6148-8-141
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author Czerwinski, Sarah L
Lyon, Andrew W
Skorobohach, Brian
Léguillette, Renaud
author_facet Czerwinski, Sarah L
Lyon, Andrew W
Skorobohach, Brian
Léguillette, Renaud
author_sort Czerwinski, Sarah L
collection PubMed
description BACKGROUND: Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoassay analysis. RESULTS: The mean tobramycin concentrations in the tears at 5, 10, 15, 30 minutes and 1, 2, 4, 6 hours after administration were 759 (±414), 489 (±237), 346 (±227), 147 (±264), 27.6 (±28.4), 14.8 (±66.6), 6.7 (±18.6), and 23.4 (±73.4) mg/L. Mean tobramycin concentration was maintained above the MIC(90) for commonly isolated bacteria for 68.5 min. CONCLUSION: A single dose of topical tobramycin resulted in therapeutic concentrations of tobramycin in the tears for 1 h after administration. Therapeutic levels of tobramycin remained in equine tears 6 times longer than was reported in rabbit tears.
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spelling pubmed-34895622012-11-06 Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay Czerwinski, Sarah L Lyon, Andrew W Skorobohach, Brian Léguillette, Renaud BMC Vet Res Research Article BACKGROUND: Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoassay analysis. RESULTS: The mean tobramycin concentrations in the tears at 5, 10, 15, 30 minutes and 1, 2, 4, 6 hours after administration were 759 (±414), 489 (±237), 346 (±227), 147 (±264), 27.6 (±28.4), 14.8 (±66.6), 6.7 (±18.6), and 23.4 (±73.4) mg/L. Mean tobramycin concentration was maintained above the MIC(90) for commonly isolated bacteria for 68.5 min. CONCLUSION: A single dose of topical tobramycin resulted in therapeutic concentrations of tobramycin in the tears for 1 h after administration. Therapeutic levels of tobramycin remained in equine tears 6 times longer than was reported in rabbit tears. BioMed Central 2012-08-21 /pmc/articles/PMC3489562/ /pubmed/22909398 http://dx.doi.org/10.1186/1746-6148-8-141 Text en Copyright ©2012 Czerwinski et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Czerwinski, Sarah L
Lyon, Andrew W
Skorobohach, Brian
Léguillette, Renaud
Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
title Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
title_full Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
title_fullStr Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
title_full_unstemmed Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
title_short Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
title_sort pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489562/
https://www.ncbi.nlm.nih.gov/pubmed/22909398
http://dx.doi.org/10.1186/1746-6148-8-141
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