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Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay
BACKGROUND: Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoa...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489562/ https://www.ncbi.nlm.nih.gov/pubmed/22909398 http://dx.doi.org/10.1186/1746-6148-8-141 |
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author | Czerwinski, Sarah L Lyon, Andrew W Skorobohach, Brian Léguillette, Renaud |
author_facet | Czerwinski, Sarah L Lyon, Andrew W Skorobohach, Brian Léguillette, Renaud |
author_sort | Czerwinski, Sarah L |
collection | PubMed |
description | BACKGROUND: Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoassay analysis. RESULTS: The mean tobramycin concentrations in the tears at 5, 10, 15, 30 minutes and 1, 2, 4, 6 hours after administration were 759 (±414), 489 (±237), 346 (±227), 147 (±264), 27.6 (±28.4), 14.8 (±66.6), 6.7 (±18.6), and 23.4 (±73.4) mg/L. Mean tobramycin concentration was maintained above the MIC(90) for commonly isolated bacteria for 68.5 min. CONCLUSION: A single dose of topical tobramycin resulted in therapeutic concentrations of tobramycin in the tears for 1 h after administration. Therapeutic levels of tobramycin remained in equine tears 6 times longer than was reported in rabbit tears. |
format | Online Article Text |
id | pubmed-3489562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34895622012-11-06 Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay Czerwinski, Sarah L Lyon, Andrew W Skorobohach, Brian Léguillette, Renaud BMC Vet Res Research Article BACKGROUND: Ophthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoassay analysis. RESULTS: The mean tobramycin concentrations in the tears at 5, 10, 15, 30 minutes and 1, 2, 4, 6 hours after administration were 759 (±414), 489 (±237), 346 (±227), 147 (±264), 27.6 (±28.4), 14.8 (±66.6), 6.7 (±18.6), and 23.4 (±73.4) mg/L. Mean tobramycin concentration was maintained above the MIC(90) for commonly isolated bacteria for 68.5 min. CONCLUSION: A single dose of topical tobramycin resulted in therapeutic concentrations of tobramycin in the tears for 1 h after administration. Therapeutic levels of tobramycin remained in equine tears 6 times longer than was reported in rabbit tears. BioMed Central 2012-08-21 /pmc/articles/PMC3489562/ /pubmed/22909398 http://dx.doi.org/10.1186/1746-6148-8-141 Text en Copyright ©2012 Czerwinski et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Czerwinski, Sarah L Lyon, Andrew W Skorobohach, Brian Léguillette, Renaud Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
title | Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
title_full | Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
title_fullStr | Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
title_full_unstemmed | Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
title_short | Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
title_sort | pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489562/ https://www.ncbi.nlm.nih.gov/pubmed/22909398 http://dx.doi.org/10.1186/1746-6148-8-141 |
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