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Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae
Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) defici...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489728/ https://www.ncbi.nlm.nih.gov/pubmed/23139841 http://dx.doi.org/10.1371/journal.pone.0049269 |
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author | Mora, Gabriel Scharnewski, Michael Fulda, Martin |
author_facet | Mora, Gabriel Scharnewski, Michael Fulda, Martin |
author_sort | Mora, Gabriel |
collection | PubMed |
description | Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) deficient cells, unable to recycle FA derived from lipid deacylation, to evaluate the role of several enzymatic activities in FA trafficking and PL homeostasis in Saccharomyces cerevisiae. The data presented show that phospholipases B are not contributing to constitutive PL deacylation and are therefore unlikely to be involved in PL remodeling. In contrast, the enzymes of neutral lipid (NL) synthesis and mobilization are central mediators of FA trafficking. The phospholipid:DAG acyltransferase (PDAT) Lro1p has a substantial effect on FA release and on PL equilibrium, emerging as an important mediator in PL remodeling. The acyl-CoA dependent biosynthetic activities of NL metabolism are also involved in PL homeostasis through active modulation of the substrate available for PL synthesis. In addition TAG mobilization makes an important contribution, especially in cells from stationary phase, to FA availability. Beyond its well-established role in the formation of a storage pool, NL metabolism could play a crucial role as a mechanism to uncouple the pools of PL and acyl-CoAs from each other and thereby to allow independent regulation of each one. |
format | Online Article Text |
id | pubmed-3489728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34897282012-11-08 Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae Mora, Gabriel Scharnewski, Michael Fulda, Martin PLoS One Research Article Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) deficient cells, unable to recycle FA derived from lipid deacylation, to evaluate the role of several enzymatic activities in FA trafficking and PL homeostasis in Saccharomyces cerevisiae. The data presented show that phospholipases B are not contributing to constitutive PL deacylation and are therefore unlikely to be involved in PL remodeling. In contrast, the enzymes of neutral lipid (NL) synthesis and mobilization are central mediators of FA trafficking. The phospholipid:DAG acyltransferase (PDAT) Lro1p has a substantial effect on FA release and on PL equilibrium, emerging as an important mediator in PL remodeling. The acyl-CoA dependent biosynthetic activities of NL metabolism are also involved in PL homeostasis through active modulation of the substrate available for PL synthesis. In addition TAG mobilization makes an important contribution, especially in cells from stationary phase, to FA availability. Beyond its well-established role in the formation of a storage pool, NL metabolism could play a crucial role as a mechanism to uncouple the pools of PL and acyl-CoAs from each other and thereby to allow independent regulation of each one. Public Library of Science 2012-11-05 /pmc/articles/PMC3489728/ /pubmed/23139841 http://dx.doi.org/10.1371/journal.pone.0049269 Text en © 2012 Mora et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mora, Gabriel Scharnewski, Michael Fulda, Martin Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae |
title | Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae
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title_full | Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae
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title_fullStr | Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae
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title_full_unstemmed | Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae
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title_short | Neutral Lipid Metabolism Influences Phospholipid Synthesis and Deacylation in Saccharomyces cerevisiae
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title_sort | neutral lipid metabolism influences phospholipid synthesis and deacylation in saccharomyces cerevisiae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489728/ https://www.ncbi.nlm.nih.gov/pubmed/23139841 http://dx.doi.org/10.1371/journal.pone.0049269 |
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