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Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata
BACKGROUND: A plant’s endogenous clock (circadian clock) entrains physiological processes to light/dark and temperature cycles. Forward and reverse genetic approaches in Arabidopsis have revealed the mechanisms of the circadian clock and its components in the genome. Similar approaches have been use...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489836/ https://www.ncbi.nlm.nih.gov/pubmed/23006446 http://dx.doi.org/10.1186/1471-2229-12-172 |
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author | Yon, Felipe Seo, Pil-Joon Ryu, Jae Yong Park, Chung-Mo Baldwin, Ian T Kim, Sang-Gyu |
author_facet | Yon, Felipe Seo, Pil-Joon Ryu, Jae Yong Park, Chung-Mo Baldwin, Ian T Kim, Sang-Gyu |
author_sort | Yon, Felipe |
collection | PubMed |
description | BACKGROUND: A plant’s endogenous clock (circadian clock) entrains physiological processes to light/dark and temperature cycles. Forward and reverse genetic approaches in Arabidopsis have revealed the mechanisms of the circadian clock and its components in the genome. Similar approaches have been used to characterize conserved clock elements in several plant species. A wild tobacco, Nicotiana attenuata has been studied extensively to understand responses to biotic or abiotic stress in the glasshouse and also in their native habitat. During two decades of field experiment, we observed several diurnal rhythmic traits of N. attenuata in nature. To expand our knowledge of circadian clock function into the entrainment of traits important for ecological processes, we here report three core clock components in N. attenuata. RESULTS: Protein similarity and transcript accumulation allowed us to isolate orthologous genes of the core circadian clock components, LATE ELONGATED HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION 1/PSEUDO-RESPONSE REGULATOR 1 (TOC1/PRR1), and ZEITLUPE (ZTL). Transcript accumulation of NaLHY peaked at dawn and NaTOC1 peaked at dusk in plants grown under long day conditions. Ectopic expression of NaLHY and NaZTL in Arabidopsis resulted in elongated hypocotyl and late-flowering phenotypes. Protein interactions between NaTOC1 and NaZTL were confirmed by yeast two-hybrid assays. Finally, when NaTOC1 was silenced in N. attenuata, late-flowering phenotypes under long day conditions were clearly observed. CONCLUSIONS: We identified three core circadian clock genes in N. attenuata and demonstrated the functional and biochemical conservation of NaLHY, NaTOC1, and NaZTL. |
format | Online Article Text |
id | pubmed-3489836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34898362012-11-06 Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata Yon, Felipe Seo, Pil-Joon Ryu, Jae Yong Park, Chung-Mo Baldwin, Ian T Kim, Sang-Gyu BMC Plant Biol Research Article BACKGROUND: A plant’s endogenous clock (circadian clock) entrains physiological processes to light/dark and temperature cycles. Forward and reverse genetic approaches in Arabidopsis have revealed the mechanisms of the circadian clock and its components in the genome. Similar approaches have been used to characterize conserved clock elements in several plant species. A wild tobacco, Nicotiana attenuata has been studied extensively to understand responses to biotic or abiotic stress in the glasshouse and also in their native habitat. During two decades of field experiment, we observed several diurnal rhythmic traits of N. attenuata in nature. To expand our knowledge of circadian clock function into the entrainment of traits important for ecological processes, we here report three core clock components in N. attenuata. RESULTS: Protein similarity and transcript accumulation allowed us to isolate orthologous genes of the core circadian clock components, LATE ELONGATED HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION 1/PSEUDO-RESPONSE REGULATOR 1 (TOC1/PRR1), and ZEITLUPE (ZTL). Transcript accumulation of NaLHY peaked at dawn and NaTOC1 peaked at dusk in plants grown under long day conditions. Ectopic expression of NaLHY and NaZTL in Arabidopsis resulted in elongated hypocotyl and late-flowering phenotypes. Protein interactions between NaTOC1 and NaZTL were confirmed by yeast two-hybrid assays. Finally, when NaTOC1 was silenced in N. attenuata, late-flowering phenotypes under long day conditions were clearly observed. CONCLUSIONS: We identified three core circadian clock genes in N. attenuata and demonstrated the functional and biochemical conservation of NaLHY, NaTOC1, and NaZTL. BioMed Central 2012-09-25 /pmc/articles/PMC3489836/ /pubmed/23006446 http://dx.doi.org/10.1186/1471-2229-12-172 Text en Copyright ©2012 Yon et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yon, Felipe Seo, Pil-Joon Ryu, Jae Yong Park, Chung-Mo Baldwin, Ian T Kim, Sang-Gyu Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata |
title | Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata |
title_full | Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata |
title_fullStr | Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata |
title_full_unstemmed | Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata |
title_short | Identification and characterization of circadian clock genes in a native tobacco, Nicotiana attenuata |
title_sort | identification and characterization of circadian clock genes in a native tobacco, nicotiana attenuata |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489836/ https://www.ncbi.nlm.nih.gov/pubmed/23006446 http://dx.doi.org/10.1186/1471-2229-12-172 |
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