Phasor Fluorescence Lifetime Microscopy of Free and Protein-Bound NADH Reveals Neural Stem Cell Differentiation Potential

In the stem cell field there is a lack of non invasive and fast methods to identify stem cell’s metabolic state, differentiation state and cell-lineage commitment. Here we describe a label-free method that uses NADH as an intrinsic biomarker and the Phasor approach to Fluorescence Lifetime microscop...

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Detalles Bibliográficos
Autores principales: Stringari, Chiara, Nourse, Jamison L., Flanagan, Lisa A., Gratton, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489895/
https://www.ncbi.nlm.nih.gov/pubmed/23144844
http://dx.doi.org/10.1371/journal.pone.0048014

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489895/
https://www.ncbi.nlm.nih.gov/pubmed/23144844
http://dx.doi.org/10.1371/journal.pone.0048014

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