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Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
Patch clamp analyses of the voltage-gated channels in sensory hair cells isolated from a variety of species have been described previously(1-4) but this video represents the first application of those techniques to hair cells from zebrafish. Here we demonstrate a method to isolate healthy, intact ha...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490287/ https://www.ncbi.nlm.nih.gov/pubmed/23117747 http://dx.doi.org/10.3791/4281 |
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author | Einarsson, Rachel Haden, Marshall DiCiolli, Gabrielle Lim, Andrea Mah-Ginn, Kolina Aguilar, Kathleen Yazejian, Lucy Yazejian, Bruce |
author_facet | Einarsson, Rachel Haden, Marshall DiCiolli, Gabrielle Lim, Andrea Mah-Ginn, Kolina Aguilar, Kathleen Yazejian, Lucy Yazejian, Bruce |
author_sort | Einarsson, Rachel |
collection | PubMed |
description | Patch clamp analyses of the voltage-gated channels in sensory hair cells isolated from a variety of species have been described previously(1-4) but this video represents the first application of those techniques to hair cells from zebrafish. Here we demonstrate a method to isolate healthy, intact hair cells from all of the inner ear end-organs: saccule, lagena, utricle and semicircular canals. Further, we demonstrate the diversity in hair cell size and morphology and give an example of the kinds of patch clamp recordings that can be obtained. The advantage of the use of this zebrafish model system over others stems from the availability of zebrafish mutants that affect both hearing and balance. In combination with the use of transgenic lines and other techniques that utilize genetic analysis and manipulation, the cell isolation and electrophysiological methods introduced here should facilitate greater insight into the roles hair cells play in mediating these sensory modalities. |
format | Online Article Text |
id | pubmed-3490287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34902872012-11-09 Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish Einarsson, Rachel Haden, Marshall DiCiolli, Gabrielle Lim, Andrea Mah-Ginn, Kolina Aguilar, Kathleen Yazejian, Lucy Yazejian, Bruce J Vis Exp Neuroscience Patch clamp analyses of the voltage-gated channels in sensory hair cells isolated from a variety of species have been described previously(1-4) but this video represents the first application of those techniques to hair cells from zebrafish. Here we demonstrate a method to isolate healthy, intact hair cells from all of the inner ear end-organs: saccule, lagena, utricle and semicircular canals. Further, we demonstrate the diversity in hair cell size and morphology and give an example of the kinds of patch clamp recordings that can be obtained. The advantage of the use of this zebrafish model system over others stems from the availability of zebrafish mutants that affect both hearing and balance. In combination with the use of transgenic lines and other techniques that utilize genetic analysis and manipulation, the cell isolation and electrophysiological methods introduced here should facilitate greater insight into the roles hair cells play in mediating these sensory modalities. MyJove Corporation 2012-10-17 /pmc/articles/PMC3490287/ /pubmed/23117747 http://dx.doi.org/10.3791/4281 Text en Copyright © 2012, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Neuroscience Einarsson, Rachel Haden, Marshall DiCiolli, Gabrielle Lim, Andrea Mah-Ginn, Kolina Aguilar, Kathleen Yazejian, Lucy Yazejian, Bruce Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish |
title | Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish |
title_full | Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish |
title_fullStr | Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish |
title_full_unstemmed | Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish |
title_short | Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish |
title_sort | patch clamp recordings in inner ear hair cells isolated from zebrafish |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490287/ https://www.ncbi.nlm.nih.gov/pubmed/23117747 http://dx.doi.org/10.3791/4281 |
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