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Protein Ligation in Living Cells Using Sortase

Sortagging is a versatile method for site-specific modification of proteins as applied to a variety of in vitro reactions. Here, we explore possibilities of adapting the sortase method for use in living cells. For intracellular sortagging, we employ the Ca(2+)-independent sortase A transpeptidase (S...

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Detalles Bibliográficos
Autores principales:	Strijbis, Karin, Spooner, Eric, Ploegh, Hidde L
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 2012
Materias:	Toolbox
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490390/ https://www.ncbi.nlm.nih.gov/pubmed/22348280 http://dx.doi.org/10.1111/j.1600-0854.2012.01345.x

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