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Effect of exercise on chemically-induced colitis in adiponectin deficient mice

BACKGROUND: Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis. METHO...

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Autores principales: Saxena, Arpit, Fletcher, Emma, Larsen, Bianca, Baliga, Manjeshwar Shrinath, Durstine, J Larry, Fayad, Raja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490765/
https://www.ncbi.nlm.nih.gov/pubmed/22909126
http://dx.doi.org/10.1186/1476-9255-9-30
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author Saxena, Arpit
Fletcher, Emma
Larsen, Bianca
Baliga, Manjeshwar Shrinath
Durstine, J Larry
Fayad, Raja
author_facet Saxena, Arpit
Fletcher, Emma
Larsen, Bianca
Baliga, Manjeshwar Shrinath
Durstine, J Larry
Fayad, Raja
author_sort Saxena, Arpit
collection PubMed
description BACKGROUND: Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis. METHODS: Male adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water. RESULTS: The clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED. CONCLUSIONS: Exercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis.
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spelling pubmed-34907652012-11-07 Effect of exercise on chemically-induced colitis in adiponectin deficient mice Saxena, Arpit Fletcher, Emma Larsen, Bianca Baliga, Manjeshwar Shrinath Durstine, J Larry Fayad, Raja J Inflamm (Lond) Research BACKGROUND: Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis. METHODS: Male adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water. RESULTS: The clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED. CONCLUSIONS: Exercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis. BioMed Central 2012-08-21 /pmc/articles/PMC3490765/ /pubmed/22909126 http://dx.doi.org/10.1186/1476-9255-9-30 Text en Copyright ©2012 Saxena et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Saxena, Arpit
Fletcher, Emma
Larsen, Bianca
Baliga, Manjeshwar Shrinath
Durstine, J Larry
Fayad, Raja
Effect of exercise on chemically-induced colitis in adiponectin deficient mice
title Effect of exercise on chemically-induced colitis in adiponectin deficient mice
title_full Effect of exercise on chemically-induced colitis in adiponectin deficient mice
title_fullStr Effect of exercise on chemically-induced colitis in adiponectin deficient mice
title_full_unstemmed Effect of exercise on chemically-induced colitis in adiponectin deficient mice
title_short Effect of exercise on chemically-induced colitis in adiponectin deficient mice
title_sort effect of exercise on chemically-induced colitis in adiponectin deficient mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490765/
https://www.ncbi.nlm.nih.gov/pubmed/22909126
http://dx.doi.org/10.1186/1476-9255-9-30
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