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Effect of exercise on chemically-induced colitis in adiponectin deficient mice
BACKGROUND: Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis. METHO...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490765/ https://www.ncbi.nlm.nih.gov/pubmed/22909126 http://dx.doi.org/10.1186/1476-9255-9-30 |
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author | Saxena, Arpit Fletcher, Emma Larsen, Bianca Baliga, Manjeshwar Shrinath Durstine, J Larry Fayad, Raja |
author_facet | Saxena, Arpit Fletcher, Emma Larsen, Bianca Baliga, Manjeshwar Shrinath Durstine, J Larry Fayad, Raja |
author_sort | Saxena, Arpit |
collection | PubMed |
description | BACKGROUND: Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis. METHODS: Male adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water. RESULTS: The clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED. CONCLUSIONS: Exercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis. |
format | Online Article Text |
id | pubmed-3490765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34907652012-11-07 Effect of exercise on chemically-induced colitis in adiponectin deficient mice Saxena, Arpit Fletcher, Emma Larsen, Bianca Baliga, Manjeshwar Shrinath Durstine, J Larry Fayad, Raja J Inflamm (Lond) Research BACKGROUND: Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis. METHODS: Male adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water. RESULTS: The clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED. CONCLUSIONS: Exercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis. BioMed Central 2012-08-21 /pmc/articles/PMC3490765/ /pubmed/22909126 http://dx.doi.org/10.1186/1476-9255-9-30 Text en Copyright ©2012 Saxena et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Saxena, Arpit Fletcher, Emma Larsen, Bianca Baliga, Manjeshwar Shrinath Durstine, J Larry Fayad, Raja Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
title | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
title_full | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
title_fullStr | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
title_full_unstemmed | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
title_short | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
title_sort | effect of exercise on chemically-induced colitis in adiponectin deficient mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490765/ https://www.ncbi.nlm.nih.gov/pubmed/22909126 http://dx.doi.org/10.1186/1476-9255-9-30 |
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