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Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
BACKGROUND: Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490825/ https://www.ncbi.nlm.nih.gov/pubmed/22894733 http://dx.doi.org/10.1186/1756-0500-5-439 |
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author | Heinälä, Pekka Lahti, Tuuli Sinclair, David Ariniemi, Kari Lillsunde, Pirjo Alho, Hannu |
author_facet | Heinälä, Pekka Lahti, Tuuli Sinclair, David Ariniemi, Kari Lillsunde, Pirjo Alho, Hannu |
author_sort | Heinälä, Pekka |
collection | PubMed |
description | BACKGROUND: Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid chromatography (HPLC). FINDINGS: Liquid-liquid extraction with butyl acetate from basic solutions (pH 9) was chosen for extraction with nalorphine as an internal standard (IS). Analytes were back-extracted from organic solvent into perchloric acid. The acid extract was chromatographed by HPLC with a reverse-phase ODS-column and electrochemical detector. The mobile phase was a NaH(2)PO(4)-solution with acetonitrile as an organic modifier and octanesulphonic acid and tetraethylammonium hydrogen sulphate as ion-pair reagents. The recovery of the extraction method was 48% for naltrexone and 75% for 6-β-naltrexol. The limit of quantification was 5.0 ng/ml for naltrexone and 1.0 ng/ml for 6-β-naltrexol. The analysed concentrations of naltrexone differed from the theoretic concentrations by 0.7 to 2.3% and those of 6-β-naltrexol by 2.6%. The relative standard deviation of within-day assay was from 0.9 to 5.7% for naltrexone and from 0.8 to 4.2% for 6-β-naltrexol; for the between-day assay it was 5.7% and 4.2%, respectively. CONCLUSIONS: Our results indicate that the developed method is suitable for determination of naltrexone and 6-β-naltrexol in human serum. |
format | Online Article Text |
id | pubmed-3490825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34908252012-11-07 Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography Heinälä, Pekka Lahti, Tuuli Sinclair, David Ariniemi, Kari Lillsunde, Pirjo Alho, Hannu BMC Res Notes Technical Note BACKGROUND: Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid chromatography (HPLC). FINDINGS: Liquid-liquid extraction with butyl acetate from basic solutions (pH 9) was chosen for extraction with nalorphine as an internal standard (IS). Analytes were back-extracted from organic solvent into perchloric acid. The acid extract was chromatographed by HPLC with a reverse-phase ODS-column and electrochemical detector. The mobile phase was a NaH(2)PO(4)-solution with acetonitrile as an organic modifier and octanesulphonic acid and tetraethylammonium hydrogen sulphate as ion-pair reagents. The recovery of the extraction method was 48% for naltrexone and 75% for 6-β-naltrexol. The limit of quantification was 5.0 ng/ml for naltrexone and 1.0 ng/ml for 6-β-naltrexol. The analysed concentrations of naltrexone differed from the theoretic concentrations by 0.7 to 2.3% and those of 6-β-naltrexol by 2.6%. The relative standard deviation of within-day assay was from 0.9 to 5.7% for naltrexone and from 0.8 to 4.2% for 6-β-naltrexol; for the between-day assay it was 5.7% and 4.2%, respectively. CONCLUSIONS: Our results indicate that the developed method is suitable for determination of naltrexone and 6-β-naltrexol in human serum. BioMed Central 2012-08-15 /pmc/articles/PMC3490825/ /pubmed/22894733 http://dx.doi.org/10.1186/1756-0500-5-439 Text en Copyright ©2012 Heinälä et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Technical Note Heinälä, Pekka Lahti, Tuuli Sinclair, David Ariniemi, Kari Lillsunde, Pirjo Alho, Hannu Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
title | Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
title_full | Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
title_fullStr | Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
title_full_unstemmed | Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
title_short | Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
title_sort | analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography |
topic | Technical Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490825/ https://www.ncbi.nlm.nih.gov/pubmed/22894733 http://dx.doi.org/10.1186/1756-0500-5-439 |
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