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Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography

BACKGROUND: Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid c...

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Autores principales: Heinälä, Pekka, Lahti, Tuuli, Sinclair, David, Ariniemi, Kari, Lillsunde, Pirjo, Alho, Hannu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490825/
https://www.ncbi.nlm.nih.gov/pubmed/22894733
http://dx.doi.org/10.1186/1756-0500-5-439
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author Heinälä, Pekka
Lahti, Tuuli
Sinclair, David
Ariniemi, Kari
Lillsunde, Pirjo
Alho, Hannu
author_facet Heinälä, Pekka
Lahti, Tuuli
Sinclair, David
Ariniemi, Kari
Lillsunde, Pirjo
Alho, Hannu
author_sort Heinälä, Pekka
collection PubMed
description BACKGROUND: Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid chromatography (HPLC). FINDINGS: Liquid-liquid extraction with butyl acetate from basic solutions (pH 9) was chosen for extraction with nalorphine as an internal standard (IS). Analytes were back-extracted from organic solvent into perchloric acid. The acid extract was chromatographed by HPLC with a reverse-phase ODS-column and electrochemical detector. The mobile phase was a NaH(2)PO(4)-solution with acetonitrile as an organic modifier and octanesulphonic acid and tetraethylammonium hydrogen sulphate as ion-pair reagents. The recovery of the extraction method was 48% for naltrexone and 75% for 6-β-naltrexol. The limit of quantification was 5.0 ng/ml for naltrexone and 1.0 ng/ml for 6-β-naltrexol. The analysed concentrations of naltrexone differed from the theoretic concentrations by 0.7 to 2.3% and those of 6-β-naltrexol by 2.6%. The relative standard deviation of within-day assay was from 0.9 to 5.7% for naltrexone and from 0.8 to 4.2% for 6-β-naltrexol; for the between-day assay it was 5.7% and 4.2%, respectively. CONCLUSIONS: Our results indicate that the developed method is suitable for determination of naltrexone and 6-β-naltrexol in human serum.
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spelling pubmed-34908252012-11-07 Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography Heinälä, Pekka Lahti, Tuuli Sinclair, David Ariniemi, Kari Lillsunde, Pirjo Alho, Hannu BMC Res Notes Technical Note BACKGROUND: Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid chromatography (HPLC). FINDINGS: Liquid-liquid extraction with butyl acetate from basic solutions (pH 9) was chosen for extraction with nalorphine as an internal standard (IS). Analytes were back-extracted from organic solvent into perchloric acid. The acid extract was chromatographed by HPLC with a reverse-phase ODS-column and electrochemical detector. The mobile phase was a NaH(2)PO(4)-solution with acetonitrile as an organic modifier and octanesulphonic acid and tetraethylammonium hydrogen sulphate as ion-pair reagents. The recovery of the extraction method was 48% for naltrexone and 75% for 6-β-naltrexol. The limit of quantification was 5.0 ng/ml for naltrexone and 1.0 ng/ml for 6-β-naltrexol. The analysed concentrations of naltrexone differed from the theoretic concentrations by 0.7 to 2.3% and those of 6-β-naltrexol by 2.6%. The relative standard deviation of within-day assay was from 0.9 to 5.7% for naltrexone and from 0.8 to 4.2% for 6-β-naltrexol; for the between-day assay it was 5.7% and 4.2%, respectively. CONCLUSIONS: Our results indicate that the developed method is suitable for determination of naltrexone and 6-β-naltrexol in human serum. BioMed Central 2012-08-15 /pmc/articles/PMC3490825/ /pubmed/22894733 http://dx.doi.org/10.1186/1756-0500-5-439 Text en Copyright ©2012 Heinälä et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Heinälä, Pekka
Lahti, Tuuli
Sinclair, David
Ariniemi, Kari
Lillsunde, Pirjo
Alho, Hannu
Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
title Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
title_full Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
title_fullStr Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
title_full_unstemmed Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
title_short Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
title_sort analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490825/
https://www.ncbi.nlm.nih.gov/pubmed/22894733
http://dx.doi.org/10.1186/1756-0500-5-439
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