Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors

Cells with monocyte/macrophage lineage expressing receptor activator of NF-κB (RANK) differentiate into osteoclasts following stimulation with the RANK ligand (RANKL). Cell adhesion signaling is also required for osteoclast differentiation from precursors. However, details of the mechanism by which...

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Autores principales: Mochizuki, Ayako, Takami, Masamichi, Miyamoto, Yoichi, Nakamaki, Tsuyoshi, Tomoyasu, Shigeru, Kadono, Yuho, Tanaka, Sakae, Inoue, Tomio, Kamijo, Ryutaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490906/
https://www.ncbi.nlm.nih.gov/pubmed/23139818
http://dx.doi.org/10.1371/journal.pone.0048795
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author Mochizuki, Ayako
Takami, Masamichi
Miyamoto, Yoichi
Nakamaki, Tsuyoshi
Tomoyasu, Shigeru
Kadono, Yuho
Tanaka, Sakae
Inoue, Tomio
Kamijo, Ryutaro
author_facet Mochizuki, Ayako
Takami, Masamichi
Miyamoto, Yoichi
Nakamaki, Tsuyoshi
Tomoyasu, Shigeru
Kadono, Yuho
Tanaka, Sakae
Inoue, Tomio
Kamijo, Ryutaro
author_sort Mochizuki, Ayako
collection PubMed
description Cells with monocyte/macrophage lineage expressing receptor activator of NF-κB (RANK) differentiate into osteoclasts following stimulation with the RANK ligand (RANKL). Cell adhesion signaling is also required for osteoclast differentiation from precursors. However, details of the mechanism by which cell adhesion signals induce osteoclast differentiation have not been fully elucidated. To investigate the participation of cell adhesion signaling in osteoclast differentiation, mouse bone marrow-derived macrophages (BMMs) were used as osteoclast precursors, and cultured on either plastic cell culture dishes (adherent condition) or the top surface of semisolid methylcellulose gel loaded in culture tubes (non-adherent condition). BMMs cultured under the adherent condition differentiated into osteoclasts in response to RANKL stimulation. However, under the non-adherent condition, the efficiency of osteoclast differentiation was markedly reduced even in the presence of RANKL. These BMMs retained macrophage characteristics including phagocytic function and gene expression profile. Lipopolysaccharide (LPS) and tumor necrosis factor –αTNF-α activated the NF-κB-mediated signaling pathways under both the adherent and non-adherent conditions, while RANKL activated the pathways only under the adherent condition. BMMs highly expressed RANK mRNA and protein under the adherent condition as compared to the non-adherent condition. Also, BMMs transferred from the adherent to non-adherent condition showed downregulated RANK expression within 24 hours. In contrast, transferring those from the non-adherent to adherent condition significantly increased the level of RANK expression. Moreover, interruption of cell adhesion signaling by echistatin, an RGD-containing disintegrin, decreased RANK expression in BMMs, while forced expression of either RANK or TNFR-associated factor 6 (TRAF6) in BMMs induced their differentiation into osteoclasts even under the non-adherent condition. These results suggest that cell adhesion signaling regulates RANK expression in osteoclast precursors.
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spelling pubmed-34909062012-11-08 Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors Mochizuki, Ayako Takami, Masamichi Miyamoto, Yoichi Nakamaki, Tsuyoshi Tomoyasu, Shigeru Kadono, Yuho Tanaka, Sakae Inoue, Tomio Kamijo, Ryutaro PLoS One Research Article Cells with monocyte/macrophage lineage expressing receptor activator of NF-κB (RANK) differentiate into osteoclasts following stimulation with the RANK ligand (RANKL). Cell adhesion signaling is also required for osteoclast differentiation from precursors. However, details of the mechanism by which cell adhesion signals induce osteoclast differentiation have not been fully elucidated. To investigate the participation of cell adhesion signaling in osteoclast differentiation, mouse bone marrow-derived macrophages (BMMs) were used as osteoclast precursors, and cultured on either plastic cell culture dishes (adherent condition) or the top surface of semisolid methylcellulose gel loaded in culture tubes (non-adherent condition). BMMs cultured under the adherent condition differentiated into osteoclasts in response to RANKL stimulation. However, under the non-adherent condition, the efficiency of osteoclast differentiation was markedly reduced even in the presence of RANKL. These BMMs retained macrophage characteristics including phagocytic function and gene expression profile. Lipopolysaccharide (LPS) and tumor necrosis factor –αTNF-α activated the NF-κB-mediated signaling pathways under both the adherent and non-adherent conditions, while RANKL activated the pathways only under the adherent condition. BMMs highly expressed RANK mRNA and protein under the adherent condition as compared to the non-adherent condition. Also, BMMs transferred from the adherent to non-adherent condition showed downregulated RANK expression within 24 hours. In contrast, transferring those from the non-adherent to adherent condition significantly increased the level of RANK expression. Moreover, interruption of cell adhesion signaling by echistatin, an RGD-containing disintegrin, decreased RANK expression in BMMs, while forced expression of either RANK or TNFR-associated factor 6 (TRAF6) in BMMs induced their differentiation into osteoclasts even under the non-adherent condition. These results suggest that cell adhesion signaling regulates RANK expression in osteoclast precursors. Public Library of Science 2012-11-06 /pmc/articles/PMC3490906/ /pubmed/23139818 http://dx.doi.org/10.1371/journal.pone.0048795 Text en © 2012 Mochizuki et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mochizuki, Ayako
Takami, Masamichi
Miyamoto, Yoichi
Nakamaki, Tsuyoshi
Tomoyasu, Shigeru
Kadono, Yuho
Tanaka, Sakae
Inoue, Tomio
Kamijo, Ryutaro
Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors
title Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors
title_full Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors
title_fullStr Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors
title_full_unstemmed Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors
title_short Cell Adhesion Signaling Regulates RANK Expression in Osteoclast Precursors
title_sort cell adhesion signaling regulates rank expression in osteoclast precursors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490906/
https://www.ncbi.nlm.nih.gov/pubmed/23139818
http://dx.doi.org/10.1371/journal.pone.0048795
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