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NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana

BACKGROUND: Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homologous-end-joining activities to produce dicentric chromo...

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Autores principales: Najdekrova, Lucie, Siroky, Jiri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490983/
https://www.ncbi.nlm.nih.gov/pubmed/22985462
http://dx.doi.org/10.1186/1471-2229-12-167
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author Najdekrova, Lucie
Siroky, Jiri
author_facet Najdekrova, Lucie
Siroky, Jiri
author_sort Najdekrova, Lucie
collection PubMed
description BACKGROUND: Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homologous-end-joining activities to produce dicentric chromosomes. During cell divisions, particularly during anaphase, dicentrics can break, thus producing naked chromosome tips susceptible to additional unwanted chromosome fusion. Many telomere-building protein complexes are associated with telomeres to ensure their proper capping function. It has been found however, that a number of repair complexes also contribute to telomere stability. RESULTS: We used Arabidopsis thaliana to study the possible functions of the DNA repair subunit, NBS1, in telomere homeostasis using knockout nbs1 mutants. The results showed that although NBS1-deficient plants were viable, lacked any sign of developmental aberration and produced fertile seeds through many generations upon self-fertilisation, plants also missing the functional telomerase (double mutants), rapidly, within three generations, displayed severe developmental defects. Cytogenetic inspection of cycling somatic cells revealed a very early onset of massive genome instability. Molecular methods used for examining the length of telomeres in double homozygous mutants detected much faster telomere shortening than in plants deficient in telomerase gene alone. CONCLUSIONS: Our findings suggest that NBS1 acts in concert with telomerase and plays a profound role in plant telomere renewal.
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spelling pubmed-34909832012-11-07 NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana Najdekrova, Lucie Siroky, Jiri BMC Plant Biol Research Article BACKGROUND: Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homologous-end-joining activities to produce dicentric chromosomes. During cell divisions, particularly during anaphase, dicentrics can break, thus producing naked chromosome tips susceptible to additional unwanted chromosome fusion. Many telomere-building protein complexes are associated with telomeres to ensure their proper capping function. It has been found however, that a number of repair complexes also contribute to telomere stability. RESULTS: We used Arabidopsis thaliana to study the possible functions of the DNA repair subunit, NBS1, in telomere homeostasis using knockout nbs1 mutants. The results showed that although NBS1-deficient plants were viable, lacked any sign of developmental aberration and produced fertile seeds through many generations upon self-fertilisation, plants also missing the functional telomerase (double mutants), rapidly, within three generations, displayed severe developmental defects. Cytogenetic inspection of cycling somatic cells revealed a very early onset of massive genome instability. Molecular methods used for examining the length of telomeres in double homozygous mutants detected much faster telomere shortening than in plants deficient in telomerase gene alone. CONCLUSIONS: Our findings suggest that NBS1 acts in concert with telomerase and plays a profound role in plant telomere renewal. BioMed Central 2012-09-17 /pmc/articles/PMC3490983/ /pubmed/22985462 http://dx.doi.org/10.1186/1471-2229-12-167 Text en Copyright ©2012 Najdekrova and Siroky; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Najdekrova, Lucie
Siroky, Jiri
NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana
title NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana
title_full NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana
title_fullStr NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana
title_full_unstemmed NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana
title_short NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana
title_sort nbs1 plays a synergistic role with telomerase in the maintenance of telomeres in arabidopsis thaliana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490983/
https://www.ncbi.nlm.nih.gov/pubmed/22985462
http://dx.doi.org/10.1186/1471-2229-12-167
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