Enhanced top-down characterization of histone post-translational modifications

Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tand...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Zhixin, Tolić, Nikola, Zhao, Rui, Moore, Ronald J, Hengel, Shawna M, Robinson, Errol W, Stenoien, David L, Wu, Si, Smith, Richard D, Paša-Tolić, Ljiljana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491414/
https://www.ncbi.nlm.nih.gov/pubmed/23034525
http://dx.doi.org/10.1186/gb-2012-13-10-r86
Descripción
Sumario:Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 µg purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.

Ejemplares similares